Ursodeoxycholic Acid Analysis Service

Ursodeoxycholic acid (UDCA) is a secondary bile acid originally isolated from bear bile, hence its name. It is commonly found in mammals and is produced through the conversion of primary bile acids by intestinal microbiota. The presence of the 7β-hydroxyl group sets UDCA apart from other bile acids, giving it unique chemical properties and biological functions. UDCA is widely used to treat liver and gallbladder diseases, such as cholestasis, primary biliary cirrhosis, and gallstones, due to its ability to reduce cholesterol absorption, promote cholesterol dissolution, protect liver cells, and alleviate bile stasis.

 

Analyzing UDCA is essential for understanding its role in disease treatment, assessing its therapeutic effectiveness, and monitoring bile acid metabolism during therapy. UDCA also plays a significant role in drug development, bile acid metabolism research, and exploring potential applications in other health areas.

 

Figure 1. Molecular Structure of Ursodeoxycholic Acid

 

MtoZ Biolabs offers Ursodeoxycholic Acid Analysis Service using high performance liquid chromatography-mass spectrometry (HPLC-MS). This advanced method combines the separation capabilities of HPLC with the detection power of MS, ensuring high sensitivity and specificity in UDAC detection.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity

Detects very low concentrations of UDCA accurately.

 

2. High Specificity

Differentiates UDCA from other compounds using mass spectrometry.

 

3. Rapid Analysis

Efficient sample preparation and fast detection processes for high-throughput analysis.

 

4. High Accuracy

Strict quality control ensures accuracy and reproducibility.

 

5. Versatility

Capable of analyzing UDCA and its related metabolites.

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, feces, tissue extracts, and other biological samples.

 

2. Sample Volume

At least 200 μL of liquid sample or 200 mg of solid sample.

 

3. Sample Preservation

Store at -80°C to maintain stability.

 

Note: Provide details on sample collection and handling.

 

Applications

• Investigate UDCA metabolic changes in the treatment of cholestasis, primary biliary cirrhosis, and gallstones, assessing its therapeutic efficacy.
• Evaluate the impact of new drugs on UDCA metabolism, aiding in new drug development and efficacy verification.
• Explore UDCA's role in bile acid metabolic pathways and potential applications in other health areas.
• Monitor changes in UDCA and its metabolites during liver and gallbladder disease treatment to optimize therapy.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Ursodeoxycholic Acid

4. Raw Data

5. Custom Analysis Report

 

MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into UDAC's extensive physiological roles through top-tier analytical techniques and expert services.