Transmission Electron Microscopy (TEM) Analytical Service

MtoZ Biolabs is a contract research organization (CRO) specializing in high-precision analytical technologies and multi-omics research, backed by extensive expertise in cutting-edge instrumentation and advanced methodologies. Transmission Electron Microscopy (TEM), renowned for its exceptional resolution and versatile imaging modes, has become an indispensable tool in life sciences and biomedical research. To address the growing need for nanoscale observations and subcellular-level analyses, MtoZ Biolabs has introduced specialized Transmission Electron Microscopy (TEM) Analytical Service designed to deliver professional, precise, and efficient sample detection and data interpretation.

 

Technical Principles  

TEM directs a high-energy electron beam through the sample, where it scatters internally, producing an image on a detector or fluorescent screen. Because electrons have extremely short wavelengths, TEM can achieve resolutions hundreds of times greater than those of optical microscopes. Common modes include conventional transmission, contrast-enhanced, and electron diffraction imaging, each tailored to specific research goals such as tissue sections, nano-composites, or viral particles. Biological specimens typically undergo fixation, dehydration, embedding, ultrathin sectioning, and heavy metal staining to enhance contrast. With state-of-the-art sample preparation technologies and a highly trained team, MtoZ Biolabs ensures ideal conditions for superior image clarity.

 



Munir, N. et al. Environ Sci Pollut Res Int. 2021.

Figure 1. Working Principle of Transmission Electron Microscopy (TEM)

 

Analysis Workflow  

• Sample Preparation and Consultation: We thoroughly discuss research objectives, sample types, desired resolution, and target areas, offering expert guidance on optimal handling procedures.  

• Pre-treatment: Depending on the material—tissue, cells, particles, or composites—we use fixation, dehydration, embedding, or negative staining. For sensitive structures, low-temperature methods help preserve original morphology.  

• TEM Parameter Configuration and Imaging: Based on sample attributes and client requirements, we select appropriate acceleration voltages, beam intensities, illumination settings, and contrast-enhancement techniques to achieve high-resolution, high-quality images.  

• Data Acquisition and Image Processing: We employ specialized software to remove noise, adjust brightness and contrast, and apply pseudo-coloring, ensuring that images meet high research standards.  

• Data Interpretation and Reporting: After processing, our team evaluates morphological features, spatial distribution, and possible structural insights, culminating in a comprehensive report to guide the next steps in research.

 

Service Advantages  

• Professional Expertise: With extensive nanoscale research experience, our seasoned TEM operators can accurately assess a variety of biological samples and microstructures.  

• High Throughput and Flexibility: We accommodate both large-scale and customized projects, adjusting protocols to balance efficiency with specific research requirements.  

• Rigorous Quality Standards: From sample intake to imaging and final analysis, our Transmission Electron Microscopy (TEM) Analytical Service follows strict quality control procedures, guaranteeing consistent, reliable outcomes.  

• Full-spectrum Technical Support: Drawing on MtoZ Biolabs’ broader capabilities in multi-omics and advanced analytics, we integrate data across platforms for deeper molecular insights.  

• Controlled Turnaround Times: We strive to expedite project completion without compromising quality and promptly provide key findings to our clients.

 

Applications  

• Proteomics and Structural Biology: Examining protein complexes or aggregates to investigate their roles in cellular processes.  

• Viruses and Pathogenic Microorganisms: Rapidly locating and classifying viral particles or bacterial cells to support infectious disease studies and therapeutic innovation.  

• Cellular Morphology and Organelle Analysis: Attaining high-resolution visualization of mitochondria, endoplasmic reticula, Golgi apparatuses, and other subcellular structures to assess cellular function.  

• Drug and Delivery Systems: Observing the stability and uniformity of liposomes, polymeric micelles, and other carrier systems for pharmaceutical development.

 

Case Study

1. Comprehensive Characterization of NPs and Protein Coronas by Transmission Electron Microscopy

 



Ashkarran, AA. et al. Nat Commun. 2022.

Figure 2. Transmission Electron Microscopy Characterization

 

2. A Flat Embedding Method for Transmission Electron Microscopy Reveals an Unknown Mechanism of Tetracycline

 



Wenzel, M. et al. Commun Biol. 2021.

Figure 3. Transmission Electron Micrographs of Flat-Embedded Bacillus subtilis, Escherichia coli, Mycobacterium bovis BCG, and Acholeplasma laidlawii

 

FAQ  

Q1. Sample Thickness Requirements  

A: Samples must be ultrathin, usually under 100 nm. We offer sectioning, grinding, and staining services to meet these specifications.

 

Q2. Potential Sample Damage  

A: TEM operates under high vacuum and uses electron beams that can affect sensitive specimens. We optimize parameters based on sample characteristics to minimize damage.  

 

Q3. Contents of the TEM Report  

A: Reports include original images, imaging parameters, structural annotations, analytical results, and recommended uses, ready for direct incorporation into research or technical applications.  

 

Q4. Typical Project Timeline  

A: Standard projects take approximately five to ten business days. For larger sample volumes or more complex requests, we finalize the schedule based on project discussions.

 

As a critical technique for microstructural analysis across diverse scientific disciplines, Transmission Electron Microscopy (TEM) underpins numerous discoveries. By combining advanced platforms, experienced researchers, and standardized procedures, MtoZ Biolabs provides high-quality Transmission Electron Microscopy (TEM) Analytical Service for academic and industrial R&D. Whether you aim to explore structural details or visualize biological specimens, we deliver robust, science-driven results. For project inquiries or technical assistance, contact MtoZ Biolabs for personalized support.