Total Amino Acid Analysis Service

Published: January 10, 2025

Total amino acid analysis is a fundamental biological assay widely utilized in the study of protein composition, nutritional status, and disease mechanisms. Total amino acid analysis provides both qualitative and quantitative information on all amino acids present in a sample, including those bound within peptide chains as well as free amino acids. By offering a comprehensive amino acid profile, This method delivers detailed proteomic insights that are essential for understanding the composition and metabolic state of complex biological samples.

MtoZ Biolabs offers advanced Total Amino Acid Analysis Service leveraging cutting-edge chromatographic separation and mass spectrometry technologies. Our services deliver precise, reliable results for a wide range of biological samples, supporting both foundational research and clinical diagnostics.

Services at MtoZ Biolabs

At MtoZ Biolabs, our Total Amino Acid Analysis Service provides precise identification and quantification of over 100 amino acids in your samples. Below is an example of the amino acid types we can detect, covering a broad spectrum to provide comprehensive analytical support for your research.

Table 1. List of Amino Acids Detecteble by MtoZ Biolabs

NO.	Metabolite	NO.	Metabolite	NO.	Metabolite
1	L-Histidine	43	2-Aminoethanesulfonic Acid	85	Glycyl-L-Proline
2	L-Arginine	44	L-Sarcosine	86	Trimethylamine N-Oxide
3	L-Serine	45	1,3-Dimethyluric Acid	87	1,3,7-Trimethyluric Acid
4	Glycine	46	N-Propionylglycine	88	3-Hydroxyhippuric Acid
5	L-Asparagine	47	N-Isovaleroylglycine	89	N8-Acetylspermidine
6	L-Glutamic Acid	48	Succinic Acid	90	(S)-β-Aminoisobutyric Acid
7	L-Threonine	49	5-Hydroxy-Tryptophan	91	S-Sulfo-L-Cysteine
8	L-Alanine	50	3,7-Dimethyluric Acid	92	Methionine Sulfoxide
9	L-Proline	51	L-Aspartate	93	Nα-Acetyl-L-Arginine
10	L-Lysine	52	L-Asparagine Anhydrous	94	1-Methylhistidine
11	L-Cystine	53	5-Hydroxy-Tryptamine	95	γ-Glutamate-Cysteine
12	L-Tyrosine	54	L-Homocitrulline	96	Nα-Acetyl-L-Glutamine
13	L-Methionine	55	Beta-Alanine	97	N-Acetyl-L-Tyrosine
14	L-Valine	56	Sarcosine	98	γ-Aminobutyric Acid
15	L-Isoleucine	57	L-Pipecolic Acid	99	D-Alanyl-D-Alanine
16	L-Leucine	58	L-Theanine	100	Guanidinoethyl Sulfonate
17	L-Phenylalanine	59	Cystathionine	101	Homo-L-Arginine
18	L-Tryptophan	60	3-N-Methyl-L-Histidine	102	L-Tryptophyl-L-glutamic Acid
19	L-Cysteine	61	Homoserine	103	Nicotinuric Acid
20	L-Glutamine	62	3-Iodo-L-Tyrosine	104	N-Acetylneuraminic Acid
21	1-Methyl-L-Histidine	63	P-Aminohippuric Acid	105	N,N-Dimethylglycine
22	3-Methyl-L-Histidine	64	4-Acetamidobutyric Acid	106	N-Acetylaspartate
23	L-Hydroxyproline	65	α-Aminoadipic Acid	107	L-Homocystine
24	O-Phosphorylethanolamine	66	D-Homocysteine	108	3-Aminoisobutanoic Acid
25	L-Carnosine	67	Trans-4-Hydroxy-L-Proline	109	Glutathione Oxidized
26	L-Anserine	68	Creatine	110	L-α-Aspartyl-L-Phenylalanine
27	Taurine	69	Kynurenine	111	N-Glycyl-L-Leucine
28	Ethanolamine	70	L-Cystathionine	112	Creatine Phosphate
29	L-Citrulline	71	5-Aminovaleric Acid	113	3-Chloro-L-Tyrosine
30	β-Alanine	72	N6-Acetyl-L-Lysine	114	S-(5-Adenosy)-L-Homocysteine
31	L-α-Aminobutyric Acid	73	Phosphorylethanolamine	115	Kynurenic Acid
32	D,L-β-Aminoisobutyric Acid	74	L-tyrosine methyl Ester	116	N'-Formylkynurenine
33	γ -Aminobutyric Acid	75	2-Aminobutyric Acid	117	Glycylphenylalanine
34	δ-D,L-Hydroxylysine	76	(5-L-Glutamyl)-L-Amino Acid	118	Argininosuccinic Acid
35	L-α-Aminoadipic Acid	77	Anserine	119	5-Hydroxylysine
36	L-Ornithine	78	6-Aminocaproic Acid	120	O-Phospho-L-Serine
37	Urea	79	Threonine	121	L-4-Hydroxyproline
38	Glutamine	80	Lysine	122	Tyrosine
39	Proline	81	Phenylalanine	123	Serine
40	Histidine	82	Ornithine	124	Valine
41	Arginine	83	Tryptophan	125	Citrulline
42	Catecholamine	84	Methionine

Note: The table above shows only a selection of the amino acids we can analyze. For other amino acid analysis services, please feel free to contact us.

Analysis Workflow

To ensure accurate and reliable results, MtoZ Biolabs' Total Amino Acid Analysis Service follows a streamlined workflow designed to maintain sample integrity and deliver precise data.

1. Sample Hydrolysis: Use acid or enzymatic hydrolysis to release amino acids from protein samples.

2. Amino Acid Separation: Separate the hydrolysis products using HPLC for precise fractionation.

3. Mass Spectrometry Analysis: Apply MS for high-precision qualitative and quantitative analysis, ensuring detailed amino acid profiles.

4. Data Processing and Reporting: Deliver customized reports with amino acid concentrations and their relative proportions within samples.

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Lam, A. K. et al. Mol Ther Methods Clin Dev. 2022.

Why Choose MtoZ Biolabs?

1. Advanced Analysis Platform: MtoZ Biolabs employs a state-of-the-art amino acid analysis platform, ensuring reliable, fast, and highly accurate results. The integration of HPLC and MS guarantees unmatched resolution and data precision.

2. Comprehensive Coverage: Our Total Amino Acid Analysis Service detects both bound and free amino acids within samples, providing a detailed and inclusive amino acid profile essential for in-depth research.

3. Scalable Throughput: Designed for efficiency, our Total Amino Acid Analysis Service processes multiple samples simultaneously, making it ideal for large-scale studies without compromising data quality.

4. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate total amino acid analysis data, providing clients with a comprehensive data report.

5. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

Sample Submission Suggestions

1. Sample Types

Serum, plasma, urine, tissue extracts, and other biological samples.

2. Sample Volume

At least 200 μL of liquid sample or 200 mg of solid sample.

3. Sample Preservation

Store samples at -20°C or lower to prevent degradation, avoiding repeated freeze-thaw cycles.

Note: Provide details on sample collection and handling.

Applications

MtoZ Biolabs' Total Amino Acid Analysis Service provides precise and comprehensive amino acid profiling, supporting diverse applications in proteomics, healthcare, nutrition, and agriculture, and driving advancements across these fields.

Proteomics: Reveal the amino acid composition of proteins to study their structure and biological functions.
Clinical Diagnostics: Monitor metabolic shifts in amino acids under various disease states to aid diagnosis.
Nutritional Science: Evaluate dietary impacts on amino acid profiles to inform personalized nutrition strategies.
Agricultural and Food Research: Assess amino acid content in agricultural and food products to improve quality and nutritional value.

Case Study

Case 1: Optimizing Dairy Product Composition Through Amino Acid Analysis

Using LC-MS/MS, this study explored the impact of different cow feeding systems (grass, clover, and concentrated feed) on the amino acid composition of skim milk powder (SMP) and whey. Dairy products from grass and clover-fed cows showed higher levels of glycine, cysteine, and valine, while concentrated feed diets resulted in increased glutamine and phosphocreatine levels. These findings highlight the importance of Total Amino Acid Analysis Service in refining feed strategies and enhancing dairy product quality.

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Magan, J. B. et al. Metabolites. 2019.

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

Our Total Amino Acid Analysis Service provides high-quality data support, advancing your scientific research and applications. For any inquiries or requirements, please contact us.

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