Total Amino Acid Analysis Service

    Total amino acid analysis is a fundamental biological assay widely utilized in the study of protein composition, nutritional status, and disease mechanisms. Total amino acid analysis provides both qualitative and quantitative information on all amino acids present in a sample, including those bound within peptide chains as well as free amino acids. By offering a comprehensive amino acid profile, This method delivers detailed proteomic insights that are essential for understanding the composition and metabolic state of complex biological samples.

     

    MtoZ Biolabs offers advanced Total Amino Acid Analysis Service leveraging cutting-edge chromatographic separation and mass spectrometry technologies. Our services deliver precise, reliable results for a wide range of biological samples, supporting both foundational research and clinical diagnostics.

     

    Services at MtoZ Biolabs

    At MtoZ Biolabs, our Total Amino Acid Analysis Service provides precise identification and quantification of over 100 amino acids in your samples. Below is an example of the amino acid types we can detect, covering a broad spectrum to provide comprehensive analytical support for your research.

     

    Table 1. List of Amino Acids Detecteble by MtoZ Biolabs

    NO.

    Metabolite

    NO.

    Metabolite

    NO.

    Metabolite

    1

    L-Histidine

    43

    2-Aminoethanesulfonic Acid

    85

    Glycyl-L-Proline

    2

    L-Arginine

    44

    L-Sarcosine

    86

    Trimethylamine N-Oxide

    3

    L-Serine

    45

    1,3-Dimethyluric Acid

    87

    1,3,7-Trimethyluric Acid

    4

    Glycine

    46

    N-Propionylglycine

    88

    3-Hydroxyhippuric Acid

    5

    L-Asparagine

    47

    N-Isovaleroylglycine

    89

    N8-Acetylspermidine

    6

    L-Glutamic Acid

    48

    Succinic Acid

    90

    (S)-β-Aminoisobutyric Acid

    7

    L-Threonine

    49

    5-Hydroxy-Tryptophan

    91

    S-Sulfo-L-Cysteine

    8

    L-Alanine

    50

    3,7-Dimethyluric Acid

    92

    Methionine Sulfoxide

    9

    L-Proline

    51

    L-Aspartate

    93

    Nα-Acetyl-L-Arginine

    10

    L-Lysine

    52

    L-Asparagine Anhydrous

    94

    1-Methylhistidine

    11

    L-Cystine

    53

    5-Hydroxy-Tryptamine

    95

    γ-Glutamate-Cysteine

    12

    L-Tyrosine

    54

    L-Homocitrulline

    96

    Nα-Acetyl-L-Glutamine

    13

    L-Methionine

    55

    Beta-Alanine

    97

    N-Acetyl-L-Tyrosine

    14

    L-Valine

    56

    Sarcosine

    98

    γ-Aminobutyric Acid

    15

    L-Isoleucine

    57

    L-Pipecolic Acid

    99

    D-Alanyl-D-Alanine

    16

    L-Leucine

    58

    L-Theanine

    100

    Guanidinoethyl Sulfonate

    17

    L-Phenylalanine

    59

    Cystathionine

    101

    Homo-L-Arginine

    18

    L-Tryptophan

    60

    3-N-Methyl-L-Histidine

    102

    L-Tryptophyl-L-glutamic Acid

    19

    L-Cysteine

    61

    Homoserine

    103

    Nicotinuric Acid

    20

    L-Glutamine

    62

    3-Iodo-L-Tyrosine

    104

    N-Acetylneuraminic Acid

    21

    1-Methyl-L-Histidine

    63

    P-Aminohippuric Acid

    105

    N,N-Dimethylglycine

    22

    3-Methyl-L-Histidine

    64

    4-Acetamidobutyric Acid

    106

    N-Acetylaspartate

    23

    L-Hydroxyproline

    65

    α-Aminoadipic Acid

    107

    L-Homocystine

    24

    O-Phosphorylethanolamine

    66

    D-Homocysteine

    108

    3-Aminoisobutanoic Acid

    25

    L-Carnosine

    67

    Trans-4-Hydroxy-L-Proline

    109

    Glutathione Oxidized

    26

    L-Anserine

    68

    Creatine

    110

    L-α-Aspartyl-L-Phenylalanine

    27

    Taurine

    69

    Kynurenine

    111

    N-Glycyl-L-Leucine

    28

    Ethanolamine

    70

    L-Cystathionine

    112

    Creatine Phosphate

    29

    L-Citrulline

    71

    5-Aminovaleric Acid

    113

    3-Chloro-L-Tyrosine

    30

    β-Alanine

    72

    N6-Acetyl-L-Lysine

    114

    S-(5-Adenosy)-L-Homocysteine

    31

    L-α-Aminobutyric Acid

    73

    Phosphorylethanolamine

    115

    Kynurenic Acid

    32

    D,L-β-Aminoisobutyric Acid

    74

    L-tyrosine methyl Ester

    116

    N'-Formylkynurenine

    33

    γ -Aminobutyric Acid

    75

    2-Aminobutyric Acid

    117

    Glycylphenylalanine

    34

    δ-D,L-Hydroxylysine

    76

    (5-L-Glutamyl)-L-Amino Acid

    118

    Argininosuccinic Acid

    35

    L-α-Aminoadipic Acid

    77

    Anserine

    119

    5-Hydroxylysine

    36

    L-Ornithine

    78

    6-Aminocaproic Acid

    120

    O-Phospho-L-Serine

    37

    Urea

    79

    Threonine

    121

    L-4-Hydroxyproline

    38

    Glutamine

    80

    Lysine

    122

    Tyrosine

    39

    Proline

    81

    Phenylalanine

    123

    Serine

    40

    Histidine

    82

    Ornithine

    124

    Valine

    41

    Arginine

    83

    Tryptophan

    125

    Citrulline

    42

    Catecholamine

     84

    Methionine

     

     

     

     Note: The table above shows only a selection of the amino acids we can analyze. For other amino acid analysis services, please feel free to contact us.

     

    Analysis Workflow

    To ensure accurate and reliable results, MtoZ Biolabs' Total Amino Acid Analysis Service follows a streamlined workflow designed to maintain sample integrity and deliver precise data.

    1. Sample Hydrolysis: Use acid or enzymatic hydrolysis to release amino acids from protein samples.

    2. Amino Acid Separation: Separate the hydrolysis products using HPLC for precise fractionation.

    3. Mass Spectrometry Analysis: Apply MS for high-precision qualitative and quantitative analysis, ensuring detailed amino acid profiles.

    4. Data Processing and Reporting: Deliver customized reports with amino acid concentrations and their relative proportions within samples.

     

    1877635856509554688-total-amino-acid-analysis-service1.jpg

    Lam, A. K. et al. Mol Ther Methods Clin Dev. 2022.

     

    Why Choose MtoZ Biolabs?

    1. Advanced Analysis Platform: MtoZ Biolabs employs a state-of-the-art amino acid analysis platform, ensuring reliable, fast, and highly accurate results. The integration of HPLC and MS guarantees unmatched resolution and data precision.

    2. Comprehensive Coverage: Our Total Amino Acid Analysis Service detects both bound and free amino acids within samples, providing a detailed and inclusive amino acid profile essential for in-depth research.

    3. Scalable Throughput: Designed for efficiency, our Total Amino Acid Analysis Service processes multiple samples simultaneously, making it ideal for large-scale studies without compromising data quality.

    4. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate total amino acid analysis data, providing clients with a comprehensive data report.

    5. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

     

    Sample Submission Suggestions

    1. Sample Types

    Serum, plasma, urine, tissue extracts, and other biological samples.

     

    2. Sample Volume

    At least 200 μL of liquid sample or 200 mg of solid sample.

     

    3. Sample Preservation

    Store samples at -20°C or lower to prevent degradation, avoiding repeated freeze-thaw cycles.

     

    Note: Provide details on sample collection and handling.

     

    Applications

    MtoZ Biolabs' Total Amino Acid Analysis Service provides precise and comprehensive amino acid profiling, supporting diverse applications in proteomics, healthcare, nutrition, and agriculture, and driving advancements across these fields.

    • Proteomics: Reveal the amino acid composition of proteins to study their structure and biological functions.
    • Clinical Diagnostics: Monitor metabolic shifts in amino acids under various disease states to aid diagnosis.
    • Nutritional Science: Evaluate dietary impacts on amino acid profiles to inform personalized nutrition strategies.
    • Agricultural and Food Research: Assess amino acid content in agricultural and food products to improve quality and nutritional value.

     

    Case Study

    Case 1: Optimizing Dairy Product Composition Through Amino Acid Analysis

    Using LC-MS/MS, this study explored the impact of different cow feeding systems (grass, clover, and concentrated feed) on the amino acid composition of skim milk powder (SMP) and whey. Dairy products from grass and clover-fed cows showed higher levels of glycine, cysteine, and valine, while concentrated feed diets resulted in increased glutamine and phosphocreatine levels. These findings highlight the importance of Total Amino Acid Analysis Service in refining feed strategies and enhancing dairy product quality.

     

    1877636431867400192-total-amino-acid-analysis-service2.png

    Magan, J. B. et al. Metabolites. 2019.

     

    What Could be Included in the Report?

    1. Comprehensive Experimental Details

    2. Materials, Instruments, and Methods

    3. Total Ion Chromatogram & Quality Control Assessment

    4. Data Analysis, Preprocessing, and Estimation

    5. Bioinformatics Analysis

    6. Raw Data Files

     

    Our Total Amino Acid Analysis Service provides high-quality data support, advancing your scientific research and applications. For any inquiries or requirements, please contact us.

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