Top-Down PTMs Analysis Service

Top-down mass spectrometry (TDMS) is highly effective for comprehensive analysis of protein post-translational modifications (PTMs) because it evaluates intact proteins or peptides without requiring hydrolysis. This method is particularly valuable in the analysis of biopharmaceutical proteins, such as monoclonal antibodies and recombinant proteins, where it preserves many unstable modifications that are often lost during collision-induced dissociation (CID) cleavage in shotgun approaches.

 



Zhang, H. et al. Circ Cardiovasc Genet. 2011.

Figure 1. Top-Down PTMs Analysis

 

Employing techniques like electron capture dissociation (ECD) and electron transfer dissociation (ETD), TDMS excels at accurately measuring the masses of whole proteins or peptides, quantifying various forms of modified proteins, creating detailed modification maps with high coverage, identifying unanticipated PTMs, and sequencing multiple modifications. Despite its relative novelty and the technical challenges it faces in sample preparation and processing throughput, top-down proteomics offers distinct benefits that render it crucial for detailed PTMs characterization.

 

At MtoZ Biolabs, our team of skilled proteomics scientists and technicians leverages extensive experience in top-down proteomics to deliver superior PTMs characterization services.