Targeted Quantitative Service
Targeted quantitative analysis is a precise method primarily used for high-sensitivity and high-specificity quantification of specific proteins, peptides, or post-translational modifications (such as phosphorylation). Unlike traditional global analysis methods, targeted quantification selectively monitors target molecules, enabling more accurate concentration data, particularly for low-abundance molecules and key biomarkers. This technology effectively addresses numerous research challenges, especially in the in-depth study of specific modification sites, disease biomarkers, and their biological relevance.
Targeted Quantitative Analysis is particularly important in phosphoproteomics, where it helps researchers explore protein post-translational modifications, particularly the role of phosphorylation in cellular functions and disease progression. At MtoZ Biolabs, we provide high-quality, high-precision Targeted Quantitative Service that supports disease research, drug development, and basic biological studies.
Technical Principles
The core of our Targeted Quantitative Service is its ability to “precisely capture” and “dynamically track” target molecules. MtoZ Biolabs employs Selected Reaction Monitoring (SRM), Multiple Reaction Monitoring (MRM), and Parallel Reaction Monitoring (PRM) technologies, combined with high-resolution mass spectrometry platforms, to achieve high-sensitivity and high-specificity targeted quantitative analysis.
SRM/MRM: This involves pre-selecting precursor-product ion pairs for target peptides, directing the collection of signals during mass spectrometry, and greatly improving sensitivity and specificity.
PRM: Based on high-resolution mass spectrometry (e.g., Orbitrap series), it captures all product ion information from target peptides in a single scan. Post-processing selects the best ion pairs, offering both high coverage and accuracy, especially for quantifying low-abundance phosphopeptides in complex samples.
Guerrero, L. et al. Metabolites. 2022.
Figure 1. Principles of SRM/MRM and PRM Technology
When combined with stable isotope-labeled internal standards (e.g., SILAC or AQUA peptides), we achieve absolute quantification, ensuring data accuracy and reproducibility in complex sample matrices.
Services at MtoZ Biolabs
Our Targeted Quantitative Service integrates end-to-end workflows to achieve high-sensitivity, high-precision analysis of phosphorylation dynamics.
1. Target Molecule Screening and Experimental Design: Helping customers select key phosphorylation sites based on research objectives and customizing detection panels.
2. Sample Preparation and Peptide Enrichment: Using TiO2/MOAC and other phosphopeptide-specific enrichment technologies along with optimized enzyme digestion methods to maximize target signal strength.
3. Mass Spectrometry Detection and Data Acquisition:
(1) SRM/MRM Mode: Using triple quadrupole mass spectrometers (e.g., Sciex Triple Quad™) for directed scanning to ensure efficient capture of low-abundance peptides.
(2) PRM Mode: Utilizing high-resolution mass spectrometers (e.g., Thermo Scientific Orbitrap Exploris™), capturing full-scan product ion information to improve quantification reliability for complex samples.
4. Data Analysis and Bioinformatics Interpretation: Using software like Skyline, MaxQuant for quantitative analysis, providing differential site screening, pathway enrichment, and visualization charts.
Why Choose MtoZ Biolabs?
1. Comprehensive Technology
Supports both SRM/MRM (high sensitivity) and PRM (high resolution) strategies, catering to different research needs.
2. Ultra-high Sensitivity and Specificity
Detection limits as low as amol levels, capable of analyzing phospho-modifications that account for less than 1% of the total protein amount.
3. Cross-species Compatibility
Supports human, mouse, plant, and other model organisms, applicable to various sample types including cells, tissues, and body fluids.
4. Experienced Team
Our highly experienced scientific team offers expert technical support and consulting services, ensuring high-quality and efficient experimental outcomes.
5. Customized Services
Tailored analysis plans and data interpretation based on customer requirements, ensuring diverse experimental needs are met.
6. One-Time-Charge
Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Suggestions
To obtain high-quality quantitative proteomics data, MtoZ Biolabs has specific requirements for sample type, quality, and preservation conditions. Common samples that can be submitted include:
Sample preservation conditions, transport methods, and buffer requirements are critical to experimental quality. MtoZ Biolabs provides detailed sample submission guidelines to ensure your samples meet optimal analysis conditions. If you have special sample types or preparation requirements, please contact our technical support team to optimize your experimental plan.
Applications
Targeted Quantitative Service delivers precise quantification of low-abundance modifications across disease research and drug development. Key applications include:
· Quantitative Phosphoproteomics: Analyzing dynamic changes in phosphorylation modifications to study cell signal transduction, metabolic regulation, and disease mechanisms.
· Cancer Research: Targeted quantitative analysis of cancer-related proteins and modification sites to provide data support for early diagnosis and tumor treatment.
· Metabolic Research: Investigating quantitative changes in key proteins and modification sites in metabolic pathways to help understand metabolic disorder mechanisms.
· Immunology Research: Analyzing quantitative changes in key proteins during immune responses, providing foundational data for immune therapy development.
· Drug Development: Evaluating the effects of candidate drugs on target proteins or modifications, supporting drug screening and mechanism research.
FAQ
Q1: How do I choose between targeted quantitative service and untargeted proteomics?
Untargeted technologies are suitable for global exploratory research, while Targeted Quantitative Service is better for longitudinal tracking of known target molecules with high sensitivity and reproducibility, making it ideal for validating candidate biomarkers or mechanism studies.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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