Targeted Mass Spectrometry
Targeted mass spectrometry is a high-sensitivity, high-specificity approach in proteomics and metabolomics, primarily used for the precise quantification of specific proteins and metabolites. Compared to conventional untargeted mass spectrometry techniques, such as data-dependent acquisition (DDA) and data-independent acquisition (DIA), targeted mass spectrometry focuses on predefined analytes, enabling reproducible and high-precision quantification in complex biological matrices.
Advantages and Applications
A major strength of targeted mass spectrometry is its selective detection capability, allowing for the precise quantification of low-abundance proteins and metabolites through optimized analytical methodologies. This technique has been extensively applied in biomedical research, clinical diagnostics, and pharmaceutical development.
1. Pharmacokinetics and Drug Metabolism
Targeted mass spectrometry plays a crucial role in pharmacokinetic (PK) studies by enabling the quantitative analysis of drug absorption, distribution, metabolism, and excretion (ADME). By generating precise pharmacokinetic profiles, this method facilitates dosage optimization, improving drug safety and therapeutic efficacy.
2. Post-Translational Modification (PTM) Analysis
Another critical application of targeted mass spectrometry is the study of post-translational modifications (PTMs), which regulate protein function, signal transduction, and the cell cycle. Modifications such as phosphorylation, acetylation, and methylation influence protein activity and interactions. Through targeted mass spectrometry, researchers can quantitatively analyze specific PTM sites, revealing dynamic regulatory mechanisms in cellular signaling pathways. PTM profiling provides new perspectives on disease pathogenesis, particularly in cancer, neurodegenerative disorders, and metabolic diseases.
Targeted Mass Spectrometry Strategies
Targeted mass spectrometry is primarily implemented through two methodologies:
1. Multiple Reaction Monitoring (MRM/SRM)
A technique commonly employed in triple quadrupole mass spectrometry, which selectively detects target molecules based on characteristic precursor-to-product ion transitions. Due to its exceptional specificity and low background interference, MRM is widely used in clinical proteomics, food safety testing, and biomarker discovery.
2. Parallel Reaction Monitoring (PRM)
A targeted quantification approach utilizing high-resolution mass spectrometry platforms such as Orbitrap and Q-TOF. Unlike MRM, PRM allows for the simultaneous detection of multiple fragment ions from target analytes, enhancing quantification accuracy and specificity. This method is particularly advantageous in PTM analysis and biological network characterization.
MtoZ Biolabs: Advanced Targeted Mass Spectrometry Services
MtoZ Biolabs offers comprehensive targeted mass spectrometry services tailored to the needs of researchers in proteomics, metabolomics, and clinical research. Leveraging state-of-the-art mass spectrometry instrumentation and bioinformatics expertise, our team delivers high-accuracy and high-reproducibility quantification solutions.
Our services encompass biomarker validation, pharmacokinetic analysis, and PTM characterization, providing researchers with reliable data to drive advancements in biomedical research, clinical translation, and precision medicine.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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