Targeted Mass Spectrometry Service

Targeted mass spectrometry is a highly precise quantitative method based on mass spectrometry that selectively measures the mass and concentration of specific target molecules, such as proteins, peptides, or metabolites, in a sample. Unlike traditional full-spectrum mass spectrometry, targeted mass spectrometry enhances sensitivity, selectivity, and quantitative accuracy by focusing on predefined ion pairs (precursor and product ions).

 

This technology has become indispensable in proteomics, metabolomics, and clinical research. Whether it's identifying disease biomarkers, evaluating drug interactions, or studying key enzymes in metabolic pathways, targeted mass spectrometry provides reliable quantitative data. It is also particularly effective in detecting low-abundance molecules, addressing the limitations of traditional analytical methods.

 

MtoZ Biolabs offers high-quality Targeted Mass Spectrometry Service Leveraging advanced mass spectrometry platforms and deep technical expertise, to support researchers in achieving groundbreaking advancements in protein quantification and molecular biology studies.

 

Technical Principles

Targeted mass spectrometry works by selectively monitoring target ion pairs for quantitative analysis. Samples are first separated using liquid chromatography (LC), followed by the isolation of specific precursor ions using mass spectrometers (e.g., quadrupoles, Orbitraps, ion traps). These ions are fragmented into product ions, and the intensity of these signals correlates with the concentration of the target molecules. Techniques like Multiple Reaction Monitoring (MRM) and Parallel Reaction Monitoring (PRM) enhance both sensitivity and selectivity.

 

Services at MtoZ Biolabs

MtoZ Biolabs offers advanced Targeted Mass Spectrometry Service utilizing cutting-edge technology to deliver high-sensitivity and high-selectivity quantitative analysis. Our services encompass a range of targeted mass spectrometry techniques, providing precise quantitative results tailored to various research needs and application scenarios. This ensures the production of high-quality, reliable experimental data. The core analysis techniques within our targeted mass spectrometry methods include the following:

 

1. Classical Targeted Mass Spectrometry Analysis

In this method, the sample is first separated using Q1 to select target precursor ions. These ions are then fragmented in Q2, generating a series of product ions. Finally, in Q3, specific product ions are selected for quantitative analysis. This approach often employs Multiple Reaction Monitoring (MRM) mode, which enables the accurate quantification of low-abundance target molecules in complex samples. It is widely used in proteomics, metabolomics, and biomarker detection.

 

2. Orbitrap Mass Spectrometry Analysis

After separation in Q1, the sample is fragmented in Q2, and the resulting ions are analyzed in an Orbitrap mass spectrometer for high-resolution analysis. Orbitrap technology provides exceptional mass resolution, making it particularly well-suited for the precise analysis of low-abundance molecules in complex samples. This method can also be coupled with Parallel Reaction Monitoring (PRM) mode, offering enhanced quantification accuracy and sensitivity, making it ideal for studies requiring high-precision quantification.

 

3. LIT and HCD Combined Analysis

This method combines Ion Trap (LIT) and High-Energy Collision Dissociation (HCD) techniques. After separation in Q1, the sample undergoes preliminary fragmentation in LIT, followed by further fragmentation using HCD. The resulting ions are then analyzed in the Orbitrap for high-resolution detection. Additionally, Tandem Mass Tags (TMT) technology can be used to label samples during the analysis, enabling the simultaneous quantification of multiple samples. This is particularly useful for high-throughput analyses and comparative studies of protein expression under different conditions.

 



Yan, Y. W. et al. Int. J. Mol. Sci. 2021.

 

Why Choose MtoZ Biolabs?

1. Advanced Analysis Platform

MtoZ Biolabs established advanced targeted mass spectrometry platform, leveraging state-of-the-art instruments like Thermo Fisher Orbitrap, QQQ mass spectrometers, and Nano-LC. This ensures fast, reliable, and highly accurate analysis across a wide range of sample types.

 

2. High Sensitivity and Selectivity

Targeted mass spectrometry effectively filters background noise, enabling accurate quantification of low-abundance molecules.

 

3. Broad Sample Compatibility

Our platform accommodates various biological samples—from purified proteins to complex matrices like cell lysates, tissues, and bodily fluids—to meet diverse research needs.

 

4. High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate alltargeted mass spectrometry data, providing clients with a comprehensive data report.

 

5. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

Case Study

Case 1: Targeted Mass Spectrometry for Early Diagnosis of Pancreatic Cancer Precursors

This study utilized targeted mass spectrometry to accurately identify cystic precursors of pancreatic cancer and uncover associated biomarkers. The results demonstrate that targeted mass spectrometry significantly improves the sensitivity and specificity of early diagnosis, providing critical support for early screening and precision therapy in pancreatic cancer. This highlights the potential application of Targeted Mass Spectrometry Service in cancer diagnostics.

 



Jabbar, K. S. ET AL. J. Clin. Oncol. 2018.

 

Case 2: Application of Targeted Mass Spectrometry in Salmonella Detection

This study successfully applied targeted mass spectrometry to detect Salmonella enterica in food samples. By targeting Salmonella-related proteins, the research showcased the high sensitivity and specificity of targeted mass spectrometry for food safety testing, offering a rapid and accurate detection method for Salmonella. This application underscores the advantages of Targeted Mass Spectrometry Service in the food safety sector, providing effective pathogen detection tools for the industry.

 



Chen, M. Q. et al. Food Chem. 2024. 

 

FAQ

Q1:What types of samples can targeted mass spectrometry analyze?

Our Targeted Mass Spectrometry Service is versatile and can handle a variety of biological samples, including serum, plasma, tissue extracts, cell lysates, urine, and more. Whether you're studying disease biomarkers, drug metabolism, or signaling pathways, we offer high-reproducibility and sensitivity tailored to your research needs.

 

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our Targeted Mass Spectrometry Service aims to provide rapid, high-throughput, and cost-effective analysis with exceptional data quality and minimal sample consumption. Contact us for a free project evaluation and more details.