Surface Plasmon Resonance (SPR) Analysis Service | Drug Discovery
Surface plasmon resonance (SPR) is a highly sensitive analytical technique widely used in the study of biomolecular interactions. SPR works by monitoring surface plasmon resonance phenomena, providing real-time detection of the dynamics and affinity of molecular interactions. Due to its label-free and real-time monitoring capabilities, SPR has become an indispensable tool in biophysics, especially in drug discovery.
Figure 1. The Basic Principle of an SPR Measurement
In the drug discovery process, SPR is mainly used to study the interactions between drug candidates and target proteins. Through SPR analysis, researchers can obtain key parameters such as the affinity, association rate, and dissociation rate of drug molecules binding to their targets. These parameters are crucial for drug optimization and screening. In addition, SPR is also used to characterize the dynamics of biomolecular interactions, helping scientists understand the mechanisms of drug action and providing data support for drug development.
MtoZ Biolabs offers high-quality Surface Plasmon Resonance (SPR) Analysis Service to global clients. Leveraging our advanced SPR technology platform, we provide efficient and precise support for drug discovery, aiding in new drug development.
Services at MtoZ Biolabs
At MtoZ Biolabs, we use SPR technology for various drug discovery-related analyses, including but not limited to drug-protein interactions, antibody-antigen interactions, and enzyme-substrate binding. Our service not only provides precise affinity data for drug screening but also helps clients analyze the association and dissociation rates between drugs and their targets, optimizing the performance of drug candidates. MtoZ Biolabs' Surface Plasmon Resonance (SPR) Analysis Service includes the following:
1. Drug-Protein Interaction Analysis: We help clients study the binding dynamics between drug molecules and target proteins, including affinity, association rate, and dissociation rate. These data are crucial for drug optimization.
2. Antibody-Antigen Interaction Analysis: Through SPR technology, we can analyze the binding characteristics between antibodies and antigens, assess antibody affinity and stability, and support antibody drug development.
3. Enzyme-Substrate Binding Analysis: SPR can be used to analyze the binding process between enzymes and substrates, revealing enzyme catalytic mechanisms and aiding in enzyme inhibitor screening.
4. Protein-Small Molecule Interaction Analysis: This service is suitable for screening small molecule drug candidates, helping to evaluate the binding capacity of small molecules with proteins and advancing the drug discovery process.
5. Comprehensive Biomolecular Dynamics Analysis: We provide a comprehensive analysis of interactions between various biomolecules, including proteins, nucleic acids, peptides, etc.
Analysis Workflow
Figure 2. The Workflow of Surface Plasmon Resonance (SPR) Analysis Service
Service Advantages
1. Advanced Platform
MtoZ Biolabs relies on an advanced SPR analysis platform combined with the latest equipment and technology to provide high-precision, high-sensitivity interaction analysis services. Our platform has powerful real-time monitoring and data processing capabilities, allowing for accurate measurement of molecular binding dynamics, affinity, and association and dissociation rates, ensuring the accuracy and reliability of the results.
2. Multi-Technology Platform Integration
MtoZ Biolabs uses a multi-technology platform to provide comprehensive and accurate interaction analysis. In addition to SPR analysis, we integrate other advanced technologies to ensure higher sensitivity and resolution. For example, when dealing with high-affinity, low-dissociation-rate interactions, we combine Isothermal Titration Calorimetry (ITC) to measure the thermodynamic properties of the binding, and X-ray Crystallography (XRC) and Nuclear Magnetic Resonance (NMR) for high-resolution structural data. For difficult-to-crystallize complex protein complexes, Cryo-Electron Microscopy can also be used as an effective complement. Through the combined use of multiple technologies, MtoZ Biolabs tailors the most suitable analysis plan for each client, ensuring that every project receives the most accurate and comprehensive results.
3. Transparent Pricing
MtoZ Biolabs is committed to providing competitive service pricing with full transparency. Throughout the service process, we will confirm the service costs with clients in advance and ensure no hidden fees. Whether for individual projects or long-term collaborations, we promise to deliver high-quality analysis at the best value, ensuring that clients receive the most cost-effective solutions.
4. Professional Team and Custom Service
MtoZ Biolabs has an experienced technical team capable of providing customized Surface Plasmon Resonance (SPR) Analysis Service based on clients' specific needs. Whether for initial screening or complex dynamic analysis, we provide professional technical support to ensure that clients achieve the best results.
Applications
Acharya, B. et al. Chem Phys Impact. 2024.
Figure 3. Different Pharmaceuticals and Biomedical Applications of SPR
FAQs
1. What types of samples can I submit for analysis?
We accept a variety of sample types, including but not limited to recombinant proteins, antibodies, small molecule compounds, and nucleic acids.
2. How long does the analysis cycle take?
The typical cycle for Surface Plasmon Resonance (SPR) Analysis Service is 1-2 weeks. The exact duration depends on sample quantity, experiment complexity, and data analysis requirements. We will provide a detailed timeline based on your specific needs and maintain communication throughout the analysis process.
Sample Submission Suggestions
1. Sample Type: We accept proteins, antibodies, small molecules, nucleic acids, and other sample types for analysis.
2. Sample Quantity: We recommend providing at least 5–10 mg of high-purity samples.
3. Sample Preparation: Please ensure the samples are free from contamination, and avoid using high salt, high concentrations of glycerol, or other substances that may interfere with the analysis.
Deliverable
1. Kinetic Data: Detailed data including association rate, dissociation rate, affinity, and other key parameters.
2. Data Report: A comprehensive analysis report including data analysis, charts, and biological interpretation.
3. Data Visualization: Clear, intuitive graphical representations of the data (e.g., curves, scatter plots) to make the results more accessible and easier to understand.
4. Recommendations & Conclusions: Based on the data analysis, we provide related recommendations and conclusions to help clients proceed with further research and optimization.
MtoZ Biolabs provides high-quality Surface Plasmon Resonance (SPR) Analysis Service to support drug discovery, antibody development, and other scientific research. If you're looking for efficient and precise biomolecular interaction analysis services, please contact us. Our professional team will provide the most suitable solutions to help drive your research forward.
Get in touch with us today to begin your collaboration!
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