Should Cells Be Lysed with RIPA Buffer Following Crosslinking in Protein Interaction Analysis?
In crosslinking-based protein interaction analysis, following treatment of cells with crosslinking reagents such as formaldehyde or disuccinimidyl suberate (DSS), cell lysis is typically performed using RIPA buffer or alternative lysis buffers. This step serves to disrupt cellular membranes and release intracellular proteins, enabling their detection in downstream analyses. RIPA buffer is particularly effective at lysing cells and tissues, facilitating protein extraction while preserving the integrity of crosslinked protein complexes.
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