S-Persulfidation Proteomics Service

MtoZ Biolabs provides S-Persulfidation Proteomics Service to identify and quantify protein S-persulfidation in complex biological samples. By combining controlled thiol handling, selective chemistries for persulfide capture, and high-resolution LC-MS analysis, we deliver site-resolved evidence and study-ready outputs for reactive sulfur species research, redox biology, and mechanism studies.

 

Overview

S-persulfidation, also known as S-perthiolation or S-sulfhydration, is a cysteine-centered modification in which a thiol (RSH) is converted to a persulfide (RSSH). Persulfides belong to the reactive sulfur species family and are increasingly recognized as biologically relevant regulators, with both low-molecular-weight forms such as glutathione persulfide and protein-bound persulfides reported in mammalian systems.

 

Functionally, S-persulfidation has been linked to changes in protein structure and activity and can be enriched under redox-perturbing conditions such as endoplasmic reticulum stress. Because persulfides are chemically reactive and susceptible to exchange during handling, reliable profiling typically requires controlled thiol preservation, selective persulfide capture or tag-switch strategies, and high-resolution LC-MS workflows that enable site-resolved identification and quantitative comparison across defined conditions.

 

S-Persulfidation Proteomics Service at MtoZ Biolabs

MtoZ Biolabs provides comprehensive S-Persulfidation Proteomics Service, including identification, quantification, and site-specific analysis of persulfidation on proteins of interest.

 

Our service employs label-free quantification, SILAC, or TMT to compare persulfidation levels across different conditions such as treatment vs. control, time courses, or genetic perturbations. This allows us to measure changes in S-persulfidation and provide insights into how these modifications influence protein function, stability, and interactions.

 

 

Figure 1. Workflow of S-Persulfidation Proteomics

 

Why Choose MtoZ Biolabs?

✅ Advanced MS Platforms
The Q Exactive HF and Orbitrap Fusion Lumos mass spectrometers offer high sensitivity and resolution, enabling detection of low-abundance persulfidated peptides.

✅ Selective Enrichment
Our enrichment methods focus on isolating persulfidated peptides, ensuring that your analysis targets the modification of interest.

✅ Comprehensive Proteomics Solutions
We provide both qualitative and quantitative data on S-persulfidation sites with detailed protein-level summaries.

✅ Multi-Sample Compatibility
Suitable for a wide range of sample types, including cell lysates, tissues, biofluids, and protein fractions.

✅ One-Time-Charge
Our pricing is transparent, no hidden fees or additional costs.

 

Sample Submission Guidelines

·  Sample types
Cultured cells, primary cells, tissues, biofluids, organoids, or protein extracts.

·  Suggested input
Provide at least 10-20 µg of protein for optimal enrichment and analysis. For limited samples, we can focus on targeted analysis.

·  Sample quality
Use fresh or frozen material, avoiding repeated freeze-thaw cycles.

·  Shipping
Ship samples on dry ice in leak-proof containers. For peptides, ship frozen and minimize moisture exposure.

For more information, please refer to Sample Submission Guidelines for Proteomics. If you are unsure about sample preparation, our team is available for consultation and can guide you through the process to ensure optimal results.

 

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

 

Start Your Project with MtoZ Biolabs

If you need reliable, site-resolved S-persulfidation data, MtoZ Biolabs is ready to support your research. Share your sample details and study goals, and we’ll design a tailored proteomics plan to suit your needs.

 

FAQ

Q1: What is the service general workflow?

 

 

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