Resources

• Principle of MS-Based Protein-Protein Interaction Analysis

Protein-protein interactions (PPIs) are fundamental to biological processes and are critical to our understanding of cellular functions. Mass spectrometry (MS) has become an indispensable tool for studying PPIs due to its high sensitivity, precision, and throughput.

• Mechanism of PCR Based Antibody Sequencing

PCR (Polymerase Chain Reaction)-based antibody sequencing methods offer efficient and sensitive capture and analysis of antibody sequences, providing profound insights into immune responses. PCR-based antibody sequencing combines antibody library cloning techniques with PCR technology. The core principle of this method involves three main steps: antibody sequence amplification, cloning, and sequencing.

• Application of PCR Based Antibody Sequencing

Antibodies are central to immune responses and are extensively used in disease detection, treatment, and research. Due to their inherent complexity and diversity, analyzing antibody sequences has been challenging.

• Workflow of PCR Based Antibody Sequencing

PCR-based antibody sequencing (PCR-Seq) has emerged as a sophisticated technique for studying antibody diversity and functionality.  

• Principle of PCR Based Antibody Sequencing

Antibodies are highly specific and diverse proteins widely utilized in biomedical research, diagnostics, and therapy. PCR-based antibody sequencing is esteemed for its efficiency, sensitivity, and accuracy. 

• Advantages and Disadvantages of PCR Based Antibody Sequencing

PCR-based antibody sequencing technology not only reveals antibody diversity and specificity but also provides robust support for antibody engineering, immune repertoire analysis, and more.

• Mechanism of De Novo Protein Sequencing

De Novo Protein Sequencing is a method for protein sequence analysis that does not rely on existing databases.

• Workflow of De Novo Protein Sequencing

Proteins are the main functional molecules within organisms, and their amino acid sequences determine their three-dimensional structures and biological functions. Therefore, deciphering the amino acid sequences of proteins is crucial for understanding their functions.  

• Application of De Novo Protein Sequencing

De novo protein sequencing does not depend on databases and can directly decode unknown protein sequences.

• Advantages and Disadvantages of De Novo Protein Sequencing

Traditional protein sequencing methods primarily rely on existing protein databases for matching and identification, but these methods have significant limitations for proteins and antibodies with unknown sequences.