Resources

Proteomics Databases



Metabolomics Databases

• • Advantages and Disadvantages of Protein Gel and Imaging Analysis

  Protein gel electrophoresis and image analysis are pivotal techniques in contemporary biological research. These methodologies are extensively utilized not only in foundational research but also in clinical diagnostics and drug discovery. This article aims to elucidate the advantages and disadvantages of protein gels and image analysis to assist researchers in optimizing their use of these technologies.

• • Principle of Protein Hydrolysis

  Protein hydrolysis is a crucial process in the fields of biology and biochemistry, involving the breakdown of proteins into their constituent amino acids or peptides. This process plays a key role in many biological functions, including digestion, cellular metabolism, signal transduction, and protein turnover. This article will delve into the basic principles of protein hydrolysis, the main types of hydrolytic enzymes, and its biological significance.

• • Principle of Protein Gel and Imaging Analysis

  Protein gel and imaging analysis technologies are pivotal in modern biological research. They are extensively used in protein separation and analysis, as well as in disease diagnosis, drug development, and basic research. This article provides a comprehensive overview of the fundamental principles of protein gel electrophoresis and imaging analysis, aiming to offer researchers a systematic knowledge background.

• • Application of Protein Sample Preparation

  Protein sample preparation is a critical step in biological and biochemical research, significantly impacting downstream analyses such as mass spectrometry, X-ray crystallography, and nuclear magnetic resonance (NMR). With advances in technology, methods for protein sample preparation have continually improved and refined.

• • Workflow of Protein Sample Preparation

  Protein sample preparation is a critical step in biological research. High-quality protein samples are essential for subsequent biochemical analyses, structural studies, and functional investigations. The following sections detail the workflow for protein sample preparation, including protein extraction, purification, quantification, and quality control.

• • Principle of Protein Sample Preparation

  Protein sample preparation is a critical step in biological research, directly impacting the accuracy and reliability of subsequent experiments. To obtain high-quality protein samples, scientists must adhere to a series of strict operational steps and principles. This article will detail the fundamental principles and related methods of protein sample preparation.

• • Differences of Labeled and Label-free Quantitative Proteomics

  Quantitative proteomics in proteinomics mainly involves two major strategies: labeled quantification and label-free quantification, which are used to compare protein abundance in different samples or under different treatment conditions.

• • Determination of Protein Disulfide Bonds

  The structure and function of proteins largely depend on their three-dimensional structure, and disulfide bonds, as one of the important covalent bonds in proteins, play a crucial role in maintaining the stability and functionality of proteins. Therefore, accurate determination of protein disulfide bonds is of great significance for understanding the structure and function of proteins.

• • Protein Phosphorylation Detection: Methods and Applications

  Protein phosphorylation is an important post-translational modification process, involving the addition of a phosphate group to a specific amino acid residue of a protein, typically serine, threonine, or tyrosine residue. Protein phosphorylation plays a critical role in regulating various biological processes within cells, including signal transduction, cell cycle, and metabolism.

• • Proteomic Identification of Human Cell Proteins

  The cell is the basic unit of life, and proteins are the key players in cell function and structure. In order to gain a deep understanding of cell function, growth, division, and their relationship with diseases, comprehensive identification and functional analysis of proteins are crucial. In this regard, mass spectrometry (MS) technology provides powerful tools for proteomics.