Resources
Proteomics Databases
Metabolomics Databases
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Environmental proteomics is an emerging interdisciplinary field that employs high-throughput techniques, such as mass spectrometry, to study the composition, function, and dynamic changes of proteins in environmental samples. This field aims to elucidate the biomolecular response mechanisms within environmental systems and their ecological and health implications. Unlike traditional genomic or metabolomic studies, environmental proteomics directly focuses on proteins, which are the functional expressi......
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Gel-based proteomics is a key method in proteomics research that employs gel electrophoresis for protein separation and analysis. This technique separates proteins based on molecular weight, isoelectric point, or charge, and integrates methods such as staining and mass spectrometry for the qualitative and quantitative analysis of proteins. One of the strengths of gel-based proteomics is its ability to visually represent the complexity of proteomes and highlight differences in protein expression throug......
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Disease proteomics is concerned with the systematic study of proteins and their expression patterns related to diseases. This field aims to uncover the mechanisms of disease occurrence and progression, as well as the characteristics of biomarkers. Unlike traditional genomic research, disease proteomics focuses on protein functions, interactions, modifications, and their roles in disease processes. Proteins, being the products of gene expression, play critical biological roles both inside and outside t......
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DIGE analysis is a powerful technique for studying variations in protein expression, based on the principles of traditional two-dimensional gel electrophoresis (2-DE). Unlike traditional methods, DIGE enhances the accuracy and efficiency of quantitative and differential protein analysis by using fluorescent dyes to label samples. This approach labels samples with distinct colors, allowing for the simultaneous separation and comparison of multiple samples on a single gel, thereby minimizing data bias f......
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DIGE 2D gel electrophoresis is a classic protein separation technique that plays a crucial role in proteomics research. Utilizing two orthogonal separation mechanisms-Isoelectric Focusing (IEF) and SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis)-this method effectively separates proteins on a two-dimensional plane. This allows for the clear analysis of thousands of proteins in complex samples. Since its inception in the 1970s, it remains an essential tool in proteomics due to its ......
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Differential proteomics analysis involves using quantitative proteomics techniques to study protein expression changes under various physiological or pathological conditions, aiming to identify proteins with significant expression differences. Unlike traditional molecular biology methods, differential proteomics provides a comprehensive view of the dynamic changes in hundreds or thousands of proteins, thus elucidating their roles in biological processes. This approach is applicable across numerous fie......
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• Differential Expression Proteomics
Differential expression proteomics involves systematically analyzing protein expression levels in various biological conditions-such as health versus disease, treated versus control groups, and different developmental stages-using advanced proteomic technologies. This approach aims to identify proteins with significantly altered expression patterns across these states. At its core, differential expression proteomics utilizes high-resolution mass spectrometry along with sophisticated bioinformatics to ......
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Diabetes proteomics employs high-throughput protein analysis techniques to investigate the protein changes associated with the onset, progression, and complications of diabetes. This approach allows for a comprehensive analysis of the differences in protein expression levels between diabetic patients and healthy individuals, thereby uncovering critical biological processes and molecular mechanisms. With the rapid advancements in mass spectrometry and bioinformatics, diabetes proteomics has become a cr......
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• DIA Proteomics Service: Advancing Disease Research
The DIA proteomics service represents an advanced mode of mass spectrometry data acquisition that has recently garnered significant attention in the field of life sciences. The choice of data acquisition methodology in proteomics research is crucial as it directly impacts the depth and precision of protein identification. Traditional DDA (Data-Dependent Acquisition) methods are limited by their reliance on predefined mass spectrometry screening criteria, which often results in the omission of low-abun......
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• Membrane Protein Mass Spectrometry
Membrane protein mass spectrometry has emerged as a significant technique in recent proteomics research, facilitating the analysis and characterization of proteins within biological membranes. Membrane proteins, which are integral to cell membranes and subcellular membranes, perform crucial roles such as signal transduction, substance transport, and cell recognition. The inherent complexity, structural diversity, and hydrophobic nature of membrane proteins present notable challenges for traditional pr......
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