Resources

Proteomics Databases



Metabolomics Databases

• • Analysis of Protein Complexes Using AP-MS

  Protein complexes play essential roles in various cellular functions, including signal transduction, metabolic regulation, and gene expression. Understanding the composition and interactions of these complexes is crucial for unraveling cellular functions and elucidating disease mechanisms. In recent years, Affinity Purification-Mass Spectrometry (AP-MS) has emerged as a powerful technique widely used in protein complex research.

• • Analysis of Protein Complexes Using Co-Immunoprecipitation and Mass Spectrometry

  Proteins typically function within cells as part of complexes, and the assembly and dynamic changes of these complexes are crucial for understanding cellular signaling, metabolic pathways, and other biological processes. The combination of immunoprecipitation (Co-IP) with mass spectrometry (MS) is a powerful technique for studying and dissecting the composition and interaction networks of protein complexes.

• • Application of PCT-DIA Proteomics

  Proteomics is an essential tool in modern life sciences for studying protein expression, function, and interactions. Among the various proteomics techniques, Data-Independent Acquisition (DIA) based methods have become increasingly popular due to their high throughput, sensitivity, and quantitative accuracy. However, as proteomics research advances, there is a growing demand for efficiency and consistency in protein sample preparation.

• • Mechanism of PCT-DIA Proteomics

  Proteomics, the field dedicated to the study of the structure, function, and interactions of proteins within a biological system, is fundamental to advancing our understanding of life sciences. The integration of Pressure Cycling Technology (PCT) with Data-Independent Acquisition (DIA) represents a significant advancement in proteomics, enhancing both the efficiency and precision of analysis.

• • Workflow of PCT-DIA Proteomics

  PCT-DIA (Pressure Cycling Technology-DIA) is an advanced analytical approach in modern proteomics. By combining Pressure Cycling Technology (PCT) with Data-Independent Acquisition (DIA), this method facilitates efficient, comprehensive, and highly sensitive analysis of complex protein samples. PCT-DIA not only improves protein identification rates but also significantly enhances the accuracy of quantitative data, establishing itself as a pivotal tool in proteomics research.

• • Advantages and Disadvantages of PCT-DIA Proteomics

  PCT-DIA (Pressure Cycling Technology-DIA) is a proteomics analysis method that combines Pressure Cycling Technology (PCT) with Data-Independent Acquisition (DIA). This technique enhances protein lysis efficiency by alternating between high and low pressures, allowing for comprehensive protein quantification using DIA.

• • Principle of PCT-DIA Proteomics

  PCT-DIA, an advanced technique in mass spectrometry, is designed to enhance the sensitivity and accuracy of protein identification and quantification. By integrating Pressure Cycling Technology (PCT) with Data-Independent Acquisition (DIA), PCT-DIA shows significant potential in proteomics research.

• • Phosphoproteomics Analysis

  Phosphoproteomics analysis is a large-scale analytic technique used to comprehensively study protein phosphorylation modifications. This analytic method can not only identify the phosphorylation sites but also quantify the changes in protein phosphorylation levels under different conditions, thereby revealing the complex regulatory mechanisms of protein functions and cellular signaling networks.

• • Construct Sequence Graph: Application of Protein Group Mass Spectrometry Analysis LC-MS/MS

  Proteins are the most basic functional molecules in biological organisms and are of great significance for understanding the structure and function of organisms. However, the complexity of proteins makes their research challenging. To solve this problem, scientists have developed a technology called proteome mass spectrometry analysis, where Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) is one of the most commonly used methods.

• • HDX Hydrogen-Deuterium Exchange in Recombinant Protein Drugs

  Recombinant protein drugs refer to protein products that are derived from animals and plants and developed through biotechnology. They have certain biological activity and can be used to prevent, treat, and diagnose diseases in humans, animals, and plants. Compared with small molecule drugs, protein drugs have advantages such as high activity, high specificity, and low toxicity, which attract a lot of researchers.