Resources

Proteomics Databases



Metabolomics Databases

• • Mechanism of Quantitative Acetylomics

  Acetylation is a crucial post-translational modification (PTM) that plays a key role in various biological processes by regulating protein activity, function, and interactions. Quantitative acetylomics aims to systematically analyze the dynamic changes of protein acetylation and reveal their functional roles in physiological and pathological conditions.

• • Application of Quantitative Acetylomics

  Acetylation is an essential post-translational modification that regulates numerous biological processes. By acetylating lysine residues on proteins, processes like cell signaling, gene expression, and metabolic regulation can be modulated. Quantitative acetylomics, a high-throughput approach, provides a systematic analysis of acetylated proteomes and their alterations under various physiological conditions.

• • Workflow of Quantitative Acetylomics

  Acetylomics is a systematic approach used to study protein acetylation modifications, widely applied to uncover protein functions, regulatory mechanisms, and their association with diseases. Quantitative acetylomics specifically focuses on the quantitative changes in protein acetylation and utilizes advanced mass spectrometry (MS) and bioinformatics to analyze the dynamic acetylation states of proteins.

• • Principle of Quantitative Acetylomics

  Acetylation is a common post-translational modification (PTM) that influences protein function, structure, and interaction by adding acetyl groups to specific sites. Dynamic changes in acetylation play a pivotal role in key cellular processes, such as gene expression, cell cycle regulation, and metabolic control.

• • Application of Post-Translational Modification Analysis

  Post-Translational Modification (PTM) refer to enzymatic or spontaneous chemical changes that occur after protein synthesis, altering the structure and function of proteins. These modifications play a pivotal role in regulating biological processes such as cell signaling, gene expression, and metabolic control. Most eukaryotic proteins undergo at least one form of PTM, highlighting their significance in both biomedicine and biotechnology.

• • Mechanism of Post-Translational Modification Analysis

  Post-translational modification (PTM) are one of the key steps in protein function regulation, widely involved in biological processes such as cell signaling, protein stability, enzymatic activity, and cell cycle regulation. The mechanisms of PTM are diverse, and different types of modifications have profound impacts on protein function, structure, and interactions with other molecules.

• • Workflow of Post-Translational Modification Analysis

  Post-translational modification (PTM) refer to the modifications of proteins after their synthesis, mediated by enzymes or other chemical reactions. These modifications play a critical role in cell signaling, regulation of protein functions, and disease mechanisms. Therefore, studying the molecular mechanisms and biological significance of PTM is a crucial step in uncovering cellular functions and pathological processes.

• • Protein Ubiquitination Site Detection Methods

  Ubiquitination is an important cellular regulatory process that involves the chemical modification of a target protein by the binding of the protein ubiquitin. This modification can regulate the stability, activity, subcellular localization, and interaction with other proteins of the target protein. Here are some common methods for detecting ubiquitination sites in proteins:

• • Protein Phosphorylation Assay

  Protein phosphorylation is a crucial cellular signaling mechanism, involving various cellular functions such as growth, division, death, and response. Phosphorylated proteins play a pivotal role in cellular signal transduction. Therefore, the study of protein phosphorylation is of great importance for understanding cell biology and disease progression.

• • Protein Modification and Detection Methods

  Protein modification is a series of chemical changes that occur in a protein after its transcription and translation. These changes play a decisive role in the function, location, and stability of the protein. There are many types of protein modifications, and the most common ones include phosphorylation, acetylation, ubiquitination, glycosylation, acylation, etc.