Resources
Proteomics Databases
Metabolomics Databases
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• Edman Degradation for Protein Sequence Analysis
Edman degradation is a classical protein sequencing technique that facilitates the sequential identification of amino acids from the N-terminus of a polypeptide chain. Developed in the 1950s by Swedish chemist Pehr Edman, this method was instrumental in the early stages of molecular biology, establishing a cornerstone for the study of protein structure and function. Edman degradation relies on the selective chemical reaction of the N-terminus of a polypeptide with phenyl isothiocyanate (PITC), formi......
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• Accurate Mass Determination in Analytical Applications
Accurate Mass Determination refers to the measurement of compound molecular mass using high-resolution mass spectrometry, characterized by exceptional precision and reliability. This technique finds extensive applications in fields such as analytical chemistry, pharmaceutical research, environmental science, and the study of macromolecules. By enabling the precise measurement of molecular mass, it facilitates detailed compound identification and structural elucidation. At its core, Accurate Mass Det......
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• Advances in Peptide Mapping Analysis for Proteomics
Peptide mapping analysis is a cornerstone technique in modern proteomics research. Renowned for its high sensitivity and resolution, this method enables comprehensive structural and functional characterization of proteins, supporting sequence confirmation, modification identification, and rigorous quality control. Its applications are increasingly critical in biopharmaceutical development, food science, and fundamental life science research. The principle of peptide mapping analysis involves enzymat......
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Lipid component analysis refers to the process of qualitatively and quantitatively analyzing lipid molecules in biological samples using scientific techniques. Lipids are essential biomolecules in living organisms, involved in critical physiological processes, including the formation of cell membranes, signal transduction, and energy storage. The core of lipid composition analysis lies in the precise identification of various lipids' types, quantities, and spatial distribution within a given sample. L......
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• Protein Interaction Network Analysis
Protein interaction network analysis is a method used to investigate the interactions between proteins in biological systems. Proteins are fundamental to life, as nearly all biological processes rely on their functions. These protein-protein interactions are crucial for the execution of these functions. Therefore, understanding the protein interaction network provides insights into the functional mechanisms of organisms. The primary role of protein interaction network analysis is to reveal the complex......
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• SDS PAGE Protein Purification
SDS page protein purification is a widely used technique in biochemistry and molecular biology for separating and analyzing individual proteins from complex mixtures. SDS page, or Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, relies on the migration of proteins in an electric field for their separation. SDS is an anionic detergent that binds non-covalently to proteins, imparting a negative charge. Additionally, SDS denatures proteins by disrupting their secondary and tertiary structures, ......
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Isotop ABPP is an innovative proteomics technique that integrates the benefits of isotope labeling, tandem mass spectrometry analysis, and protein digestion reactions to comprehensively study protein functions and activation states. In this technique, “isotop” refers to stable isotope tags used to label proteins or their fragments, providing accurate quantification in mass spectrometry analyses. The term “tandem” refers to the multi-stage process in mass spectrometry, where ions are generated through ......
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• Protein Interaction Analysis Overview
Protein Interaction Analysis aims to uncover the physical and functional relationships between proteins. Proteins, as fundamental molecules of life, form complex networks through interactions, performing various biological functions. Protein interaction analysis has broad applications across multiple fields. In drug development, understanding the interactions of specific proteins aids in identifying drug targets, thus enhancing the selectivity and efficacy of drugs. In disease research, abnormal prote......
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• Single Cell T Cell Receptor Sequencing
Single cell T cell receptor sequencing is an advanced technique used to investigate the diversity and specificity of T cells in immune responses. The T cell receptor (TCR) is a protein complex on the surface of T cells that recognizes and binds specific antigen peptides in conjunction with major histocompatibility complex (MHC) molecules, triggering T cell immune responses. The high diversity of TCRs enables them to recognize a broad array of antigens, which underpins the adaptive and specific nature ......
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Edman degradation steps are precise and efficient, primarily comprising: 1. Sample Preparation, ensuring the purity and integrity of the protein sample; 2. Chemical Modification, reacting phenylisothiocyanate (PITC) with the N-terminus of the polypeptide chain to form derivatives; 3. Selective Degradation, cleaving and releasing the labeled N-terminal amino acid; 4. Detection and Identification, identifying the released amino acids through chromatography or mass spectrometry. This series of operations......
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