Quasi-Targeted Metabolomics Analysis Service
- Biomarker discovery and validation
- Metabolic pathway research
- Drug metabolism research
- Environmental and food sciences
Quasi-Targeted metabolomics is an advanced metabolomics technology that integrates the high-throughput screening capabilities of non-targeted metabolomics with the high accuracy and sensitivity of targeted approaches. This technique allows for broad metabolite coverage while enabling precise quantification and identification of target metabolites. It is especially well-suited for monitoring metabolites in complex biological systems and conducting functional studies. Quasi-targeted metabolomics provides critical support in the areas of metabolic pathway elucidation, biomarker discovery, and drug metabolism research.
Technical Principles
At MtoZ Biolabs, we employ cutting-edge triple quadrupole mass spectrometry in combination with multiple reaction monitoring (MRM) to deliver precise metabolite detection and quantification. With our internally curated metabolite database, we can efficiently screen biological samples while ensuring accurate quantification of known metabolites. Retention time (RT), along with parent and daughter ion pair information (Q1/Q3), is used during analysis to ensure reliable qualitative assessments. Our platform is designed to cover a wide array of metabolites, while maintaining exceptional sensitivity and precision, ensuring that our clients' metabolomics research is robust and highly accurate.
Figure 1. Diagram of MRM Principle
Analysis Workflow
1. Sample Collection and Processing
Clients provide biological samples such as blood, urine, or tissue, which are processed under specific protocols to ensure metabolite stability and integrity.
2. Metabolite Database Construction
A tailored list of metabolites, including known biomarkers and target compounds, is generated based on the research objectives, enabling accurate quantification and detailed analysis.
3. LC-MS Analysis
Using liquid chromatography-mass spectrometry (LC-MS), we separate and detect metabolites in the samples with high precision, and quantification is achieved through MRM mode for targeted metabolites.
4. Data Analysis and Interpretation
Professional bioinformatics tools are utilized to process the data, quantify metabolites, and interpret results based on retention time and ion pair information, ensuring accurate identification and quantification.
5. Comprehensive Reporting
A detailed report is generated, including the types of metabolites detected, their concentrations, and related metabolic pathways, providing clients with in-depth insights into their biological systems.
Service Advantages
Why Choose MtoZ Biolabs?
1. Advance Analysis Platform: MtoZ Biolabs established an advanced metabolomics analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. Exceptional Sensitivity and Precision: Our triple quadrupole mass spectrometry platform is capable of detecting low-abundance metabolites with extraordinary sensitivity, while maintaining a high degree of reproducibility and accuracy.
3. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
4. Customized Service: Tailored to the specific research needs of our clients, we offer flexible experimental design and personalized data analysis to ensure the achievement of research goals to the fullest extent.
Applications
Deliverables
What Could be Included in the Report?
1. Comprehensive Experimental details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs leverages its state-of-the-art mass spectrometry platforms and extensive expertise in metabolomics to offer precise, high-efficiency services across targeted, non-targeted, and quasi-targeted metabolomics. We are committed to helping researchers achieve groundbreaking results in the field. For more information or a consultation, please feel free to contact us.
How to order?
