Quantitative Succinylation Service

Succinylation is a biochemical process in which a succinyl group donor (such as succinyl-CoA) covalently attaches a succinyl group to the lysine residue of a target protein via enzymatic or non-enzymatic reactions. This modification introduces two negative charges to the lysine residue, significantly altering the protein's physicochemical properties, spatial conformation, and biological functions. As a highly conserved post-translational modification, succinylation occurs extensively in both eukaryotic and prokaryotic organisms. Research indicates a close relationship between succinylation and acetylation, as many sites of succinylation also exhibit acetylation. This modification is integral to various core metabolic pathways, including the tricarboxylic acid cycle and carbohydrate metabolism, playing a critical role in the regulation of mitochondrial function. Consequently, succinylation is particularly significant in investigating diseases associated with mitochondrial dysfunction.

 

 

Kurmi, K. et al. Cell Rep. 2018.

Figure 1. The Process of Lysine Succinylation

 

MtoZ Biolabs provides Quantitative Succinylation Service that leverage advanced mass spectrometry (MS) platforms along with specific enrichment strategies, enabling the efficient isolation and quantitative analysis of succinylated proteins from biological samples. Our service utilize affinity capture in conjunction with mass spectrometry to precisely identify and quantify succinylated proteins and their modification sites, ensuring high-quality and reliable results.

 

Analysis Workflow

 

 

 

Service Advantages

1. High Sensitivity

Our services can detect and analyze low-abundance succinylated proteins, making them suitable for a wide variety of sample types.

 

2. High Throughput

We support the simultaneous analysis of multiple samples, greatly improving experimental efficiency.

 

3. Quantitative Accuracy

Our methodology employs internal standards and standard curves to guarantee the reliability of quantitative outcomes.

 

Sample Submission Requirements

 



For more sample details, please consult our technical team.

 

Applications

• Investigating core energy metabolism
• Studying mitochondrial metabolic disorders
• Analyzing inflammatory diseases, among others.

 

MtoZ Biolabs is dedicated to delivering high-quality Succinylation Quantitative Service, facilitating innovation and advancement in scientific research. We encourage potential clients to reach out to our expert team for further information!