Quantitative Enzyme Activity Analysis by ABPP and LC-MS/MS

Incubate lysates with ABPs, enrich tagged enzymes, digest, and quantify probe-modified peptides by label-free or TMT LC-MS/MS across conditions.

Key takeaways

• Only active enzymes react with warheads.
• Include competition controls.
• Probe class defines enzyme coverage.
• MS quant tracks activity changes under drug treatment.







Related services

ABPP services Activity-Based Protein Profiling (ABPP) Service ABPP Service Chemical Proteomics Analysis Service Enzyme Activity Assay Service

Target discovery Target Identification Service Drug Target Identification Service Label-Free Quantitative Proteomics Service TMT Quantitative Proteomics Service

FAQ

ABPP vs expression proteomics? ABPP reports catalytic activity, not total protein level.

What are limitations? Probe design and non-covalent enzymes need tailored strategies.

Conclusion

Quantitative ABPP connects functional enzyme states to phenotypes and compound action.