Purified Protein Post-Translational Modification Sites Identification Service

Post-translational modifications (PTMs) refer to the covalent modification of specific amino acid residues in proteins after translation, resulting in changes to their conformation, activity, or interaction properties. These modifications regulate protein stability, localization, and interactions, and have a profound impact on cellular functions such as signal transduction and metabolic regulation. Common PTMs include phosphorylation, acetylation, ubiquitination, and methylation. Identifying these modification sites is essential for understanding the regulatory mechanisms of protein function, elucidating their pathophysiological roles in biological processes, and discovering potential drug targets.

 

MtoZ Biolabs provides a Purified Protein Post-Translational Modification Site Identification Service based on advanced mass spectrometry (MS) technology. This service offers researchers precise and high-throughput identification of modification sites, enabling a deeper understanding of the functional roles of protein modifications. The service is compatible with purified proteins from various biological samples and meets the needs of both basic research and drug development.

 

Technical Principles

The identification of modification sites involves the initial purification of the target protein using techniques such as immunoprecipitation (IP), co-immunoprecipitation (Co-IP), pull-down assays, or electrophoresis. Once purified, the target protein is subjected to mass spectrometry analysis, where covalent attachment of specific modification groups leads to a measurable increase in molecular mass. By detecting these mass shifts, the mass spectrometer accurately quantifies the presence of specific modifications and identifies the corresponding modified amino acid residues.

 

Analysis Workflow

 



 

Service Advantages

1. High Accuracy

The combination of high-resolution mass spectrometry with liquid chromatography enables the highly sensitive detection and precise identification of low-abundance modification sites.

 

2. Comprehensive Modification Profiling

Supports the identification of a wide range of modification types, including phosphorylation, acetylation, methylation, and glycosylation.

 

3. Versatile Adaptability

Applicable to various modification types and protein samples, catering to both basic and applied research needs.

 

4. High-Throughput Capabilities

Capable of processing large-scale sample volumes, providing rapid and comprehensive data for research.

 

5. Quantitative Analysis

In addition to identifying modification sites, relative quantification of these sites is provided, revealing dynamic changes in modification abundance.

 

Sample Submission Requirements

1. Sample Type

Purified proteins obtained through IP, Co-IP, pull-down, or electrophoresis, with a purity >80% and concentration >1 mg/mL.

 

2. Sample Volume

A minimum of 20 μg of purified protein per sample is required. The modification types and sample processing protocols are customizable according to specific research requirements.

 

Applications

1. Signal transduction pathway research: Identification of key protein modification sites within signaling pathways to elucidate the role of modifications in signal transduction.

 

2. Disease biomarker discovery: Identification of disease-related protein modifications, facilitating the discovery of potential biomarkers.

 

3. Metabolic regulation studies: Analysis of post-translational modifications in metabolic enzymes to understand their regulatory roles in metabolic pathways.

 

4. Drug target identification and screening: Evaluation of the effects of drugs on specific protein modifications, aiding in the discovery and optimization of therapeutic targets.

 

5. Cell biology research: In-depth analysis of protein modifications involved in cell cycle regulation, apoptosis, and differentiation, advancing research in cellular biology.

 

Deliverables

What Could be Included in the Report?

1. Comprehensive Experimental details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)

4. Data Analysis, Preprocessing, and Estimation (project-dependent)

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption.