Pseudouridine Analysis Service

Pseudouridine (Ψ) is a modified nucleoside and the most abundant post-transcriptional modification in RNA, particularly in noncoding RNAs (ncRNAs) such as Transfer RNA (tRNA) and Ribosomal RNA (rRNA). It derives from uridine through the action of pseudouridine synthase enzymes. Structurally, pseudouridine differs from uridine by having a carbon-carbon instead of a nitrogen-carbon glycosidic bond. The presence of pseudouridine contributes to the stability of tRNA and rRNA structures and plays a crucial role in the function of ncRNAs. Pseudouridine plays a vital role in RNA structure and function. Alterations in pseudouridine levels can significantly impact cellular processes, leading to various diseases, including mitochondrial myopathy and sideroblastic anemia (MLASA) and dyskeratosis congenita. Pseudouridines have been identified as regulators of viral latency processes in human immunodeficiency virus (HIV) infections. Therefore, understanding the pseudouridine landscape in RNA can provide valuable insights into the pathogenesis of diseases and potential therapeutic targets.

 



 

Deb, I. et al. Sci Rep. 2019.

 

Figure 1. Chemical Structure of (a) Uridine and (b) Pseudouridine

 

MtoZ Biolabs offers a specialized Pseudouridine Analysis Service. We are committed to providing accurate, comprehensive pseudouridine analysis and interpretation using our sophisticated  liquid chromatography-mass spectrometry (LC-MS/MS) platforms and bioinformatics tools. We offer:

• Identification and quantification of pseudouridine in various RNA types.
• Profiling of pseudouridine modifications in response to different conditions or treatments.
• Analysis of pseudouridine dynamics during development or disease progression.

 

Analysis Workflow

 



 

Service Advantages

• Extensive experience in various biological sample preparation techniques
• State-of-the-art mass spectrometry platforms
• Professional standard operating procedure
• Highly sensitive and reliable analysis
• Rapid turnaround time and competitive pricing Customized service

 

Applications

Our pseudouridine analysis service is applicable to various research areas, including:

• Biomarker discovery for disease diagnosis and therapy.
• Functional studies of pseudouridine in ncRNA.
• Drug discovery and development.

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues, and other biological samples. It is recommended to prepare at least 3 biological replicates for each sample type.

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Storage and Shipping

Samples should be stored at -80℃ and shipped with dry ice. Avoid repeated cycles of freezing and thawing

 

Note: Please provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Pseudouridine

4. Raw Data

5. Custom Analysis Report

 

At MtoZ Biolabs, we strive to support your research with our advanced pseudouridine analysis service. Contact us today to discuss your project needs.