Targeted Protein Degradation Drugs Characterization Service

    With the rapid development of biotechnology, drug research and development has gradually entered a new era with precision and personalization. Targeted protein degradation (TPD) drugs are an emerging therapeutic strategy that utilizes body's ubiquitin-proteasome system (UPS) to degrade specific pathological proteins to achieve precise treatment. As an important new class of therapeutic drugs, TPD drugs are attracting more and more attention and and have inspired more innovation. The design of TPD drugs has become a hot topic in the drug research and development field.

     

    Proteomics, a science that studies the composition, structure, function, and mutual interactions of proteins within organisms, can fully reveal the interactions between drugs and proteins. By integrating proteomics, we can deep into the targeting action of TPD drugs and provide important bases for drug optimization design and clinical application.

     

    MtoZ Biolabs uses Thermo's Obitrap Fusion Lumos and other high-resolution mass spectrometers to establish a TPD drug targeted proteomics analysis platform, offering you TPD drug characteristic analysis services based on whole proteomics technology. A single experiment can identify 8000-10000 kinds of proteins. We can conduct following experiments:

     

    1. TPD Activity Analysis

    Using data independent acquisition (DIA) technology and professional protein retrieval software, it comprehensively covers the proteome and effectively improves the quantitative accuracy of the protein of onterest (POI).

     

    2. Target Selectivity Analysis

    By differential proteomic analysis of POI and other differential protein changes, We can comprehensively evaluate the targeting selectivity of TPD drugs.

     

    3. Potential Off-Target and Safety Concerns Analysis

    Based on the results of TPD drug targeting selectivity, We can integrate multi-platform database analysis to explore potential off-target risks and safety issues of TPD drugs.

     

    4. Biological Functions and Mechanisms of Action Analysis

    Using classic bioinformatics analysis strategies such as PPI, GO, and KEGG, We can explore the biological functions and mechanisms of action mediated by TPD drugs.

     

    Analysis Workflow

    1. Sample Preparation: Reasonable Concentration, Time Point Experimental Design

    2. Sample Pre-Treatment: Total Protein Extraction, Proteolysis

    3. Mass Spectrometry Collection: DIA Collection, DIANN Library Search

    4. Data Processing: Automated Analysis Workflow, Personalized Analysis Workflow

     

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    Figure 1. TPD Drug Targeted Proteomic Analysis Experimental Process

     

    Sample Submission Requirements

    1. Cell Precipitates

    At least one treatment group and one control group per set, at least three samples per group recommended.

     

    2. Tissue Samples

    At least one treatment group and one control group per set, at least five samples per group recommended.

     

    Services at MtoZ Biolabs

     

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