Protein Mutation Analysis Service

Protein mutation refers to the alteration in the amino acid composition or sequence of a protein, which subsequently leads to changes in the structure and function of the protein. Protein mutations can occur naturally or be induced artificially. The analysis of protein mutations primarily involves comparing the differences between normal proteins and mutant proteins, revealing the effects of mutations on protein structure, stability, function, and interactions with other molecules. In the fields of biotechnology and drug design, mutation analysis aids in understanding the molecular mechanisms of proteins, optimizing protein performance, predicting the impact of mutations on biological functions, providing a basis for biotechnology and drug design, and enhancing the production efficiency of bioproducts and the specificity of drug therapy.

MtoZ Biolabs provides a one-stop, mass spectrometry-based protein mutation service utilizing Thermo's newly launched Obitrap Exploris 240 mass spectrometer combined with Nano-LC chromatography technology. Through high-resolution mass spectrometry analysis of protein amino acid sequence, it reveals whether the protein has been mutated or not, the site of mutation, and the amino acid sequences before and after the mutation, which can help research on the structure and function of proteins, the regulation of gene expression, and drug discovery.

Service Advantages

1. Advanced Mass Spectrometry Technology

We employ state-of-the-art high-resolution mass spectrometers, including the Q-Exactive series and Orbitrap series, to ensure the accuracy and reliability of data. These instruments capture extensive protein feature information, providing high-resolution protein mass spectra.

2. Rich Databases and Algorithms

We utilize various protein sequence databases, such as UniProt, Swiss-Prot, etc., and advanced search algorithms such as SEQUEST, Mascot, etc., to achieve high-precision protein identification and mutation analysis.

3. High-Throughput Data Analysis Platform

We have developed a robust data analysis platform using the latest bioinformatics tools and algorithms to perform various analyses including protein identification, quantification, functional annotation, enrichment analysis, and so on.

4. Strict Quality Control

We adhere to rigorous quality control processes from sample handling to data analysis, ensuring the services we provide are highly accurate and reproducible.

5. Personalized Customized Services

We offer customized experimental designs and data analysis solutions to meet specific research objectives of different clients.

Applications

1. Discovery of New Proteins

Used for designing novel proteins to enhance specific functions or alter their structures to meet specific application requirements.

2. Study of Protein Structure and Function

Comparing protein sequences and structural differences between different species or individuals can lead to the development of computational methods to predict protein structure and function, furthering understanding of the formation and evolution mechanisms of biodiversity.

3. Molecular Evolution

Tracing the evolutionary history of organisms can elucidate the evolutionary relationships and functional evolution of different species.

4. Development of Drugs

Investigating the interactions between proteins and drugs facilitates the development of drugs targeting specific targets.

5. Diagnosis and Treatment of Disease

Used to identify protein mutations associated with certain diseases, thereby designing relevant treatment methods.

Sample Submission Requirements

*Please avoid using reagents that may interfere with mass spectrometry analysis, such as detergents, salts, and polyethylene glycol.

Deliverables

In technical reports, MtoZ Biolabs will provide detailed technical reports, including: