Sequence Mutation Analysis Service of Proteins Expressed in Stable Cell Lines

During the construction and storage of cell lines, minor sequence variations (SV) may occur in protein products, potentially altering biological activity and triggering immune responses, thus posing significant risks. Early detection and characterization of these variations are essential to ensure drug safety and efficacy. Advances in mass spectrometry (MS) technology enable the high-sensitivity detection of sequence variations. By integrating high-performance liquid chromatography (HPLC) with tandem mass spectrometry (MS/MS), we can comprehensively analyze protein sequences and identify any variations, providing precise and reliable mutation analysis, especially for recombinant proteins and cell lines.

 

The development of stable cell lines is critical for producing biotherapeutic drugs. However, unexpected amino acid substitutions in recombinant therapeutic proteins can cause sequence variations (SV) that significantly affect drug efficacy and safety. Although these variations are typically present at very low levels in optimized production systems, they can increase substantially in non-optimized cell lines or due to unexpected DNA mutations. Hence, detecting these mutations before large-scale production is vital.

 

MtoZ Biolabs offers comprehensive sequence mutation analysis service of proteins expressed in stable cell lines, leveraging de novo sequencing platforms and advanced bioinformatics tools to ensure the accurate and complete detection of all mutations in protein products under various conditions. Our platform utilizes HPLC-UV/MS/MS to characterize peptide maps from multiple protease digestions, combined with bioinformatics analysis, to identify all amino acid sequence variations.

 

Service Advantages

1. High Coverage

Achieving very broad sequence coverage using multiple protease digestions and efficient HPLC-MS/MS technology, ensuring no variations are missed.

 

2. Stability and Reproducibility

Stable cell lines can continuously express mutant proteins, which can ensure experimental stability and reproducibility.

 

3. Professional Team

Our team of scientists and technicians possesses extensive expertise in mass spectrometry and proteomics, ensuring the highest quality service.

 

4. Fast Turnaround

We provide rapid result delivery while maintaining high-quality analysis, typically completing the process in a short time.

 

Application

1. Protein Function Research

Mutation analysis can reveal how different amino acid residues in a protein sequence affect its function, helping to identify functional regions and active sites.

 

2. Signal Transduction Research

Studying mutations in key proteins within signaling pathways can help understand their roles and mechanisms in cellular signal transduction.

 

3. Disease Mechanism Research

By simulating disease-related mutations, researchers can study how these mutations lead to the onset and progression of diseases, uncovering potential pathogenic mechanisms.

 

4. Drug Target Validation

Mutation analysis can be used to confirm drug targets and assess the effects of drugs on mutant proteins.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Sequence Mutation Analysis of Proteins Expressed in Stable Cell Lines

4. Mass Spectrometry Images

5. Raw Data