Protein Mass Analysis Service
Protein mass analysis is a key component of proteomics that provides critical insights into the molecular weight, structure, and integrity of proteins. Accurate mass determination is essential for verifying recombinant protein sequences, detecting mutations and post-translational modifications, assessing protein degradation, ensuring homogeneity, and quantifying isotope incorporation during labeling.
Service at MtoZ Biolabs
MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. At MtoZ Biolabs, we offer cutting-edge Protein Mass Analysis Services, employing high-resolution mass spectrometry (MS) techniques such as LC-MS and MALDI-TOF-MS. These technologies are widely used in both research and the biopharmaceutical industry due to their precision and reliability. MALDI-TOF-MS directly measures protein molecular weight, while ESI-MS (Electrospray Ionization Mass Spectrometry) analyzes multi-charged ions and deconvolutes the data to deliver highly accurate molecular weight values. Each method has its unique advantages and is suited for different applications in protein mass analysis.
Figure1. MALDI-MS and ESI-MS procedures
Our comprehensive Protein Mass Analysis Service provides customized solutions tailored to the specific needs of your research. Whether you need to verify protein sequences, analyze peptide fingerprints, or characterize protein heterogeneity, MtoZ Biolabs ensures fast, accurate, and reliable results, supporting your discoveries and advancing your scientific goals. If you are interested in our services, please feel free to contact us.
Analysis Workflow
Service Advantages
1. Advance Analysis Platform: MtoZ Biolabs established an advanced Protein Mass Analysis Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all protein mass analysis data providing clients with a comprehensive data report.
4. Fast Turnaround: The process from sample handling to report generation is efficient, shortening the analysis cycle.
5. Customizable Service: MtoZ Biolabs provides tailored service to suit specific research needs.
Applications
1. Proteomics
Protein mass analysis is essential for characterizing recombinant proteins, identifying post-translational modifications, confirming protein sequences, and supporting structural studies in large-scale proteomics.
2. Biopharmaceuticals
In biopharmaceutical development, protein mass analysis ensures the purity, stability, and consistency of therapeutic proteins, monoclonal antibodies, and vaccines, crucial for regulatory compliance and product quality.
3. Other Applications
Beyond proteomics and biopharma, protein mass analysis is used in biomarker discovery, quality control in protein production, and environmental or agricultural research to study protein responses to various conditions.
Sample Submission Suggestions
1. Sample Form: Samples can be submitted either as dry powder or in solution form.
2. Sample Quantity: Please provide a sample size ranging from 10 to 50 µg for optimal analysis.
3. Sample Purity: Ensure the sample purity is greater than 90% for accurate results.
To ensure the integrity of the analysis, avoid using surfactants such as SDS, Triton-X, or similar compounds. Additionally, minimize the salt concentration (e.g., Tris) in the sample as much as possible.
*Notes: Feel free to contact us if you have any additional sample requirements.
Case Study
Representative MALDI-TOF mass spectra of influenza A and B infected cell cultures (“Influenza A-MDCK SIAT1 infected cells” and “Influenza B-MDCK SIAT1 infected cells”) and uninfected cell cultures (“MDCK SIAT1 control uninfected cells”).
Calderaro, A. et al. Sci Rep. 2016.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Relevant Liquid Chromatography and Mass Spectrometry Parameters
4. The Detailed Information of Protein Mass Analysis
5. Mass Spectrometry Image
6. Raw Data
How to order?
