Protein Fingerprinting Service
- Low-complexity samples: Gel spots or bands, approximately 1-2 cm in length.
- Medium-complexity samples: Protein mixtures, including column-purified samples.
- Moderate-to-high complexity samples: Protein mixtures such as IP-derived samples, secreted proteomes, or subcellular organelles (e.g., mitochondria, endoplasmic reticulum, lysosomes).
- High-complexity samples: Whole cell lysates, tissues.
- Protein Identification
- Biomarker Discovery
- Quality Control for Biopharmaceuticals
- Antibody Screening and Optimization
- Environmental and Food Safety Testing
Protein fingerprinting, also known as peptide mass fingerprinting (PMF), is a widely-used mass spectrometry technique for protein identification. First developed independently by several research groups in 1993, this method involves enzymatically cleaving proteins into peptides and measuring their mass-to-charge ratios (m/z) for rapid, precise identification. Unlike whole-genome sequencing, Protein fingerprinting does not require complete protein sequence data. Instead, it identifies proteins based solely on the peptide mass data compared to theoretical data in established databases.
MtoZ Biolabs offers highly efficient and accurate Protein Fingerprinting Service for the rapid identification and quantification of proteins. Our service is extensively applied in basic research, biomarker discovery, proteomics, and quality control for biopharmaceuticals, providing robust data support for both research and industrial applications.
Analysis Workflow
1. Sample Separation
Protein samples are first separated using 1D or 2D PAGE.
2. Digestion
Selected bands or spots are excised, and the proteins are enzymatically digested into peptides.
3. Mass Spectrometry Analysis
Peptide mass-to-charge ratios are measured using mass spectrometers such as MALDI-TOF or LC-MS/MS.
4. Data Comparison
The resulting mass spectrometry data are matched against theoretical peptide masses in a database to identify the proteins.
5. Report Generation
A comprehensive report detailing the protein identification results is provided.
Service Advantages
Why Choose MtoZ Biolabs?
1. Advance Analysis Platform: MtoZ Biolabs established an advanced protein fingerprinting platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. No Sequence Data Requirement: Complete protein sequence data is not needed; our service uses peptide mass comparison alone for accurate identification.
3. Flexible Identification Options: Quick, first-order mass spectrometry for straightforward samples, with MS/MS for enhanced precision in complex analyses.
4. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all proteomics analysis data providing clients with a comprehensive data report.
5. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Suggestions
Samples that can be tested by Protein Fingerprinting Service include:
For more specific sample submission requirements, please consult our technical team.
Applications
FAQ
Q1: Can Protein Fingerprinting Service handle complex mixtures?
This service is best suited for single proteins or simple mixtures. For samples containing multiple proteins, we recommend MS/MS analysis to achieve high accuracy and resolution. This approach allows precise identification even in complex samples. For higher-throughput needs, our LC-MS/MS service is also available as an ideal option.
Q2: If my sample has already been separated by 1D/2D PAGE, is further liquid chromatography necessary?
For samples with clear PAGE bands, we typically excise, digest, and proceed with mass spectrometry. However, for complex samples or bands with multiple protein components, our nano-LC system provides additional separation, enhancing resolution and identification accuracy. MtoZ Biolabs will select the optimal approach based on your sample to ensure reliable results.
Deliverables
What Could be Included in the Report?
1. Comprehensive Experimental details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details!
How to order?
