Protein Carboxyethylation Analysis
Protein carboxyethylation is a significant post-translational modification (PTM) where a carboxyethyl group (-CH2-COOH) is covalently added to lysine or cysteine residues, altering protein structure and function. Recent research has demonstrated that this modification generates neoantigens, inducing HLA-restricted autoimmune responses, particularly playing a crucial role in autoimmune diseases such as ankylosing spondylitis (AS).
Zhai, Y. et al. .Science. 2023.
Figure 1. Cysteine Carboxyethylation Provokes HLA-Restricted Autoimmune Responses in Ankylosing Spondylitis
MtoZ Biolabs, utilizing advanced mass spectrometry platforms and specialized proteomic techniques, provides highly sensitive and specific protein carboxyethylation detection and quantification services, facilitating the detailed investigation of this modification in both physiological and pathological contexts.
Analysis Workflow
Services at MtoZ Biolabs
1. Targeted Protein Carboxyethylation Analysis
MtoZ Biolabs offers Targeted Protein Carboxyethylation Analysis for client-specified proteins. After enzymatic digestion, carboxyethylated peptides are enriched and analyzed by high-resolution LC-MS/MS to accurately identify and quantify modification sites on the target protein.
2. Carboxyethylation Proteomics
MtoZ Biolabs provides Carboxyethylation Proteomics for global profiling of carboxyethylation across the proteome. Using large-scale LC-MS/MS with peptide-level enrichment, this service enables comprehensive mapping and quantitative assessment of carboxyethylation in complex biological samples.
Service Advantages
1. Advance Analysis Platform
MtoZ Biolabs established an advanced protein PTM analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. Wide Application
Our analysis is adaptable to numerous biological sample types, supporting the systematic study of protein carboxyethylation across diverse physiological and pathological conditions.
3. High-Data-Quality
Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate PTM proteomics analysis data providing clients with a comprehensive data report.
4. Customized Solutions
We offer tailored services, ranging from the identification of modification sites to comprehensive functional annotation, meeting the specific needs of various research projects.
5. One-Time-Charge
Our pricing is transparent, no hidden fees or additional costs.
Applications
Protein carboxyethylation has significant research potential in autoimmune and other pathological processes. MtoZ Biolabs' carboxyethylation analysis services support the following areas:
- Autoimmune Disease Research: Neoantigens generated by carboxyethylation are closely linked to HLA-restricted autoimmune responses, making this service ideal for studying molecular mechanisms in diseases like ankylosing spondylitis and rheumatoid arthritis.
- Metabolic Disorder Research: By detecting changes in carboxyethylation induced by metabolic byproducts, our services contribute to the investigation of metabolic diseases such as diabetes and atherosclerosis.
- Oxidative Stress Studies: Carboxyethylation is involved in cellular responses to oxidative damage, and its quantification aids in exploring antioxidant defense mechanisms.
- Drug Development: Monitoring carboxyethylation during drug testing allows for precise assessment of therapeutic efficacy and target engagement, supporting new drug development efforts.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details!
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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