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    Processing Proteomics Data with R

      Processing proteomics data is an important aspect of bioinformatics, and R programming language is widely used due to its powerful capabilities in statistical computation and graphical visualization. In proteomics data analysis, R can be applied to a variety of tasks, including but not limited to data preprocessing, mass spectrometry data analysis, protein identification, quantification, biomarker discovery, as well as functional and pathway analysis. Below are some R packages and resources that may be used for processing proteomics data:

       

      Bioconductor

      Bioconductor is a collection of R packages specifically designed for biological data analysis. It provides several packages for proteomics data analysis, such as MSnbase, mzR, and ProtGenerics. Bioconductor also offers comprehensive documentation and tutorials to assist users.

       

      MSnbase

      MSnbase is an R/Bioconductor package designed for mass spectrometry data analysis. It supports data import, processing, and visualization, offering an object-oriented toolkit for convenient handling and analysis of mass spectrometry data.

       

      mzR

      The mzR package provides support for reading and writing mass spectrometry files, such as mzXML, mzData, and mzML formats. It is one of the foundational tools for processing mass spectrometry data.

       

      pRoloc

      pRoloc is an R package for protein localization analysis in proteomics data. It supports quantitative mass spectrometry data and is designed to help users determine the subcellular localization of proteins.

       

      limma

      Although limma was originally developed for microarray data analysis, it can also be used for differential expression analysis of proteomics data. limma identifies proteins with significant expression differences across various conditions.

       

      ClusterProfiler

      ClusterProfiler is an R package for performing enrichment analysis of biological terms (such as GO and KEGG pathway analysis), which aids users in interpreting the biological significance of proteomics data.

       

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