Prior to Mass Spectrometry Submission, Should the Protein Gel Be Stored in Deionized Water? Will the Protein Diffuse or Degrade?
The protein gel can be stored in deionized water (ddH₂O) before submission for mass spectrometry; however, long-term storage in deionized water is not advised, as this may lead to protein dispersion into the surrounding solution or degradation. To preserve protein stability, it is recommended to select an appropriate storage method based on the specific requirements of subsequent analyses, such as short-term storage under low-temperature conditions or in a suitable buffer solution. Ideally, mass spectrometric analysis should be conducted promptly to minimize potential protein degradation.
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