Preparation of Single Cell Suspensions for Mass Cytometry

    Materials and Instruments

    1. Experimental cell lines

    2. Phosphate-buffered saline (PBS)

    3. 0.25% Trypsin-EDTA

    4. Centrifuge

    5. Flow cytometer

     

    Preparation Procedure

    1. Cell Washing and Harvesting

    Begin by washing the cells with PBS. Following this, treat the cells with 0.25% Trypsin-EDTA to facilitate cell detachment and harvesting.

     

    2. Suspension and Centrifugation

    Resuspend the detached cells in PBS. Transfer the suspension to a centrifuge tube, and centrifuge at 300g for 5 minutes to pellet the cells.

     

    3. Resuspension and Filtration

    After discarding the supernatant, resuspend the cell pellet in fresh PBS. Filter the cell suspension to remove any aggregates and large debris.

     

    4. Cell Counting and Dilution

    Use a cell counter or hemocytometer to determine the cell concentration. Adjust the cell suspension to the desired dilution according to experimental requirements.

     

    5. Preparation for Mass Spectrometry Analysis

    Prepare a single-cell suspension tailored for mass spectrometry analysis. This step may require modifications based on the specific protocols associated with the mass spectrometer in use.

     

    MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.

    Related Services

    Single Cell Mass Cytometry Analysis

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