Prenylation Analysis Service
- High Sensitivity Analysis: Utilizing the latest mass spectrometry technologies to ensure precise identification of low-abundance prenylated proteins.
- Efficient and High-Throughput: Supporting the processing of large-scale samples, ideal for high-throughput research needs.
- Diverse Applications: Widely applicable to various cell lines, animal models, and a range of research demands.
- Accurate and Reliable Data: Providing comprehensive data support to ensure reproducibility and accuracy of research findings.
Prenylation is an important post-translational modification, where isoprenoid groups (such as farnesylation or geranylgeranylation) are attached to the cysteine residues of proteins. This modification plays a crucial role in regulating protein localization, interactions, and signal transduction within the cell. MtoZ Biolabs offers specialized prenylation analysis service, assisting researchers in exploring protein farnesylation status and advancing research into related disease mechanisms.
Protein prenylation is essential for maintaining normal cellular functions. It not only helps proteins localize to the cell membrane but also regulates multiple signaling pathways, particularly the functions of key regulatory factors such as Ras family proteins. Due to its widespread role in cells, prenylation is critical in the development of several diseases, including cancer, neurodegenerative diseases, and cardiovascular diseases. In cancer, excessive farnesylation of Ras proteins often leads to uncontrolled cell proliferation. Therapeutic strategies usually focus on inhibiting the activity of related transferases or developing inhibitors to modulate the prenylation process, thereby interfering with cancer cell growth and spread. For neurodegenerative diseases, restoring normal prenylation states provides new avenues for treatment.
Palsuledesai, CC. et al. ACS Chem Biol. 2015.
Figure 1. The Process of Protein Prenylation
Combining advanced mass spectrometry techniques with customized analysis strategies, MtoZ Biolabs offers an innovative prenylation analysis service to help clients accurately identify and quantify protein farnesylation and geranylgeranylation modifications. We provide efficient, precise analysis of protein prenylation, helping you uncover its functions within cells and its potential roles in diseases.
Figure 2. The Workflow of Prenylation Analysis
Service Advantages
Case Study
1. Dual chemical probes enable quantitative system-wide analysis of protein prenylation and prenylation dynamics
Protein farnesylation and geranylgeranylation are critical post-translational modifications (PTMs) that influence the localization and function of key proteins, including Ras. This study introduces YnF and YnGG, two isoprenoid analogs used in conjunction with quantitative chemical proteomics to achieve comprehensive and selective profiling of protein modifications in living cells. A total of 80 prenylated proteins were identified in a single human cell line, with 64 newly discovered at native abundance. The research further examines the effects of four prenyltransferase inhibitors on modification dynamics and explores Rab protein prenylation defects, providing novel tools and perspectives for investigating regulatory mechanisms of protein modifications. Prenylation analysis service employs high-sensitivity mass spectrometry combined with chemical labeling to enable global, quantitative analysis of protein farnesylation. It covers modified protein identification, post-translational processing status elucidation, and dynamic tracking of modifications. The approach facilitates the evaluation of regulatory mechanisms, inhibitor action, and disease models exhibiting abnormal prenylation, delivering precise analytical support for biomedical and pharmaceutical research.
Storck, EM. et al. Nat Chem. 2019.
Figure 3. Direct Detection of Post-Translational Processing of Prenylated Peptides by LC-MS/MS
2. Dual-Stage Neutral Loss Tandem Mass Spectrometric Strategy for Confident Identification of Protein Prenylation
Protein prenylation is an important post-translational modification that regulates protein interactions, localization, and signaling pathways, which is crucial for normal eukaryotic cell function and plays a key role in the development of various cancers. Due to the high hydrophobicity of prenylated peptides and the lack of efficient enrichment methods, direct identification of prenylated proteins by mass spectrometry has been challenging. This study presents a dual-stage neutral loss MS3 (DS-NLMS3) strategy, combining the gas-phase cleavable properties of prenyl thioether bonds and oxidized prenyl thioether. This significantly improves the confidence in identifying prenylated proteins, enabling the direct identification of low-abundance prenylated peptides and native prenylated proteins from mouse macrophage cells without prior enrichment. This method provides an efficient solution for prenylation research. The prenylation analysis service uses high-sensitivity mass spectrometry combined with customized strategies to achieve accurate identification and quantification of protein prenylation. The service includes prenylation site confirmation, post-translational modification dynamics tracking, and detection of low-abundance modified peptides, enabling identification of native prenylated proteins without prior enrichment. This service offers strong analytical support for protein modification studies and proteomics research.
Fang Z, et al. Anal Chem. 2021.
Figure 4. DS-NLMS3 Strategy for the Identification of Prenylated Proteins
Service Applications
✅Cancer Research: Exploring the prenylation mechanisms of Ras proteins and other cancer-related proteins.
✅Neurodegenerative Diseases: Understanding disease mechanisms by analyzing prenylation abnormalities.
✅Drug Development: Providing experimental support for drugs targeting prenylation.
✅Protein Function Research: Investigating protein modifications and their roles across different organisms.
How Can We Help You?
1. Communication of Requirements: Fill out the inquiry form below to receive a customized solution.
2. Sample Submission: We accept various forms of samples, including liquids, lyophilized powders, and colloidal solutions. Samples should be properly packaged to prevent contamination or degradation during transportation. It is recommended that the sample concentration be maintained within an optimal range to ensure detection sensitivity and accuracy. Extremely low concentrations may lead to unstable analytical results. Samples should be stored at an appropriate temperature to avoid exposure to high temperatures or repeated freeze-thaw cycles. For degradable samples, we recommend using frozen or dry ice transportation. When submitting samples, please complete the sample submission form and provide detailed information, such as sample source, purity, and pre-treatment methods, to allow our team to develop a customized analysis plan for you.
3. Experiment and Analysis: Tailored analysis will be completed within 15 business days for routine projects, with real-time progress updates provided for complex projects.
4. Report Delivery: Reports include raw data, experimental methods, prenylation site identification, quantitative modification data, both liquid chromatography and mass spectrometry data, and bioinformatics analysis results.
Prenylation analysis service at MtoZ Biolabs utilizes precise technical approaches to support breakthroughs in biological and pharmaceutical research, driving your scientific projects toward success!
How to order?
