Premade Phage Display Antibody Library Screening Service for Exosome
Exosomes are small extracellular vesicles secreted by most cell types and are abundant in various body fluids, including blood, urine, saliva, and cerebrospinal fluid. These vesicles carry diverse bioactive molecules—such as proteins, nucleic acids, and lipids—that reflect the physiological or pathological status of their cells of origin. Due to their stability, accessibility, and molecular content, exosomes have emerged as important targets for disease diagnostics, biomarker discovery, and therapeutic development. However, selective detection or targeting of exosome subpopulations remains a technical challenge. Conventional antibody development for exosomal surface markers is time-consuming and resource-intensive. To accelerate exosome-targeted research and therapeutic applications, MtoZ Biolabs offers a specialized Premade Phage Display Antibody Library Screening Service for Exosome.
The Premade Phage Display Antibody Library Screening Service for Exosome enables high-throughput, efficient, and customized discovery of exosome-binding antibodies using high-diversity phage libraries and optimized biopanning workflows. Phage display is a robust in vitro selection technique that links phenotype (antibody binding ability) to genotype (antibody-encoding DNA) by displaying peptide or antibody fragments on the surface of bacteriophages. Through iterative rounds of selection—commonly referred to as "biopanning"—against a specific target, phage clones bearing high-affinity binders can be enriched from large combinatorial libraries. In the context of exosome research, phage display allows the direct screening of antibodies against intact, native exosomes, preserving vesicle structure and surface antigen conformation. This approach is particularly advantageous for identifying ligands that recognize conformation-dependent epitopes or membrane proteins with low abundance.
Analysis Workflow
1. Exosome Preparation
Isolate and characterize exosomes from client samples, ensuring vesicle purity and integrity.
2. Library Selection
Choose an appropriate premade phage display library based on project needs and target type.
3. Biopanning Rounds
Perform 3–5 rounds of selection against intact exosomes, incorporating blocking and negative selection to enrich for specific binders.
4. Clone Screening
Screen enriched clones by ELISA or other immunoassays to assess binding affinity and specificity.
5. Sequence Analysis
Sequence positive clones to identify unique antibody candidates with exosome-binding potential.
6. Optional Validation
Provide recombinant antibody production and functional validation upon request.
Figure 1. Types of Antibody Phage Display Libraries (Upper Left Panel), Library Construction (Lower Left Panel) and Antibody Selection (Right Panel)
Why Choose MtoZ Biolabs?
✅Ready-to-Use High-Diversity Libraries: We utilize well-validated premade phage display libraries with high diversity and broad epitope coverage, enabling rapid identification of high-affinity binders.
✅End-to-End Workflow: From exosome isolation and characterization to clone screening and sequence analysis, we provide a comprehensive solution under one roof, ensuring consistency and efficiency.
✅Experienced Technical Support: Our expert team offers in-depth consultation at every stage—from project design to data interpretation—ensuring that your screening goals are fully met.
✅One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Applications
The Premade Phage Display Antibody Library Screening Service for Exosome is suitable for a wide range of research and translational applications, including:
1. Exosome Marker Discovery
Identify novel surface markers or conformation-specific antigens on disease-associated exosomes.
2. Antibody-Based Exosome Capture
Develop antibodies for exosome enrichment from complex biofluids or culture media for proteomic or transcriptomic profiling.
3. Targeted Drug Delivery
Generate exosome-binding antibodies or antibody fragments to guide therapeutic cargo to specific vesicle subtypes.
4. Diagnostics and Liquid Biopsy Development
Isolate disease-specific exosome populations from blood or urine for biomarker detection in oncology, neurology, or infectious disease.
5. Therapeutic Antibody Development
Discover antibody candidates that neutralize or block exosome-mediated signaling pathways in tumor microenvironments or immune regulation.
FAQ
Q1: How do you reduce non-specific binding during screening?
We incorporate optimized blocking strategies and negative selection steps to minimize background binding and enrich target-specific phage clones.
Q2: What applications are supported by this service?
The Premade Phage Display Antibody Library Screening Service is ideal for exosome capture, biomarker discovery, diagnostics development, and therapeutic targeting in fields such as oncology, immunology, and regenerative medicine.
If you are interested in our Premade Phage Display Antibody Library Screening Service for Exosome, please feel free to contact us. Our technical specialists are available to provide a free business assessment.
How to order?
