Post-Translational Modification Expression Profiling Service

Post-Translational modification (PTM) expression profiling is a large-scale, qualitative shotgun mass spectrometry analysis aimed at comprehensively identifying modification sites and exploring their biological functions. Unlike traditional protein expression profiling, PTM profiling focuses on enriching modified peptides and precisely identifying modification sites. This approach provides essential insights into the role of PTMs in different physiological and pathological states.

 

Heitel, P. et al. Molecules. 2023.

Figure 1. Common Types of PTMs

 

MtoZ Biolabs offers a PTM Expression Profiling Service based on cutting-edge mass spectrometry technology. This service enables high-throughput and precise identification of protein modification sites, supporting a wide range of research from basic biology to disease biomarker discovery and drug development.

 

Analysis Workflow

 



 

Service Advantages

1. Comprehensive Qualitative Analysis

Shotgun mass spectrometry enables large-scale qualitative analysis of protein modifications, generating detailed PTM expression profiles.

 

2. Accurate Identification of Modification Sites

High-resolution mass spectrometry, combined with peptide enrichment techniques, provides precise identification of modification sites.

 

3. Multi-Modification Detection

The service supports the detection of various PTMs, including phosphorylation, acetylation, methylation, and ubiquitination.

 

4. High-throughput Analysis

Capable of processing large sample volumes efficiently, making it suitable for large-scale studies.

 

5. Comprehensive Data Interpretation

Bioinformatics tools deliver an in-depth analysis of modification sites, offering insights into the regulatory roles of PTMs.

 

Sample Submission Requirements

Sample Type: Cells, tissues, or purified proteins from various sources.

 

Sample Quantity: At least 20 μg of protein or an equivalent amount of cells or tissue.

 

Modification Type Information: Clients must provide details on the target modification type and sample information in advance to ensure the most appropriate enrichment and detection methods are used.

 

Deliverables

What Could be Included in the Report?

1. Comprehensive Experimental details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)

4. Data Analysis, Preprocessing, and Estimation (project-dependent)

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs' PTM Expression Profiling Service, leveraging advanced mass spectrometry and bioinformatics analysis, helps researchers make breakthrough progress in protein modification studies. This service provides reliable data support and in-depth analysis for studying the biological roles of PTMs. Free project evaluation, welcome to learn more details! Our technical specialists are available to provide a free business assessment.