Polarized Raman Spectroscopy Analytical Service

Polarized Raman spectroscopy is a spectroscopic analysis method that combines Raman scattering with polarization optics. Its basic principle is that when sample molecules are excited by a specific polarized laser, the polarization state of the scattered light varies with the molecular symmetry and orientation, thereby revealing molecular vibration modes, spatial configurations, and orientation information. By analyzing the intensity differences of Raman signals at different polarization angles, in-depth studies of molecular structural symmetry and orientation distribution can be achieved. This technique combines the high molecular specificity of Raman spectroscopy with the directional sensitivity of polarization optics and is widely applied in the study of molecular orientation, structural symmetry, and conformational changes, supporting microscopic characterization of protein and nucleic acid folding, polymer chain structures, as well as crystals and functional materials.

  



Zhang, X. et al. Cellulose, 2022.

Figure 1.  Schematic Diagram of the αLVR Polarization Configuration for Raman Spectroscopy

   

Services at MtoZ Biolabs

Based on an advanced polarized Raman spectroscopy platform, MtoZ Biolabs provides polarized Raman spectroscopy analytical service capable of rapidly collecting and comparing spectral signals at different polarization angles. This service enables in-depth analysis of molecular symmetry, conformational differences, and orientation distribution of samples, helping to reveal molecular structural features and local arrangement patterns. By delivering high-quality polarization-dependent spectral data and parameter results, this service is not only applicable to the study of biomacromolecular conformations but also provides reliable support for the microscopic characterization of polymers, crystals, and functional materials.

   

Analysis Workflow

1. Sample Preparation

Pretreat samples such as cells, proteins, polymers, or crystals to ensure uniformity and representativeness.

  

2. Laser Polarization Excitation

Irradiate samples with lasers at different polarization angles to induce direction-dependent Raman scattering signals.

  

3. Spectral Acquisition

Use a high-resolution polarized Raman spectrometer to record scattering spectral data under different polarization conditions.

  

4. Data Processing

Perform background correction and peak identification on the spectra, analyzing the intensity variations with polarization changes.

  

5. Result Interpretation

Output complete polarization-dependent Raman spectra and orientation parameters, revealing molecular symmetry, orientation distribution, and conformational features.

   

Sample Submission Suggestions

1. Sample Type

Applicable to a wide range of samples such as proteins, cells, tissue sections, polymers, and crystalline materials. Samples should be uniform and free from obvious cracks or impurities to ensure the reliability of polarization signals.

   

2. Sample Purity

It is recommended to remove impurities or fluorescent contaminants that may cause background interference, ensuring that Raman and polarization signals are clear and analyzable.

   

3. Sample Storage

Samples should be stored under dry, dark, and low-temperature conditions to prevent structural changes or signal distortion caused by light exposure, moisture, or high temperatures.

   

4. Sample Transport

Samples should be transported in sealed containers, with desiccants or cold-chain conditions used if necessary, to ensure stability and integrity before reaching the analytical platform.

   

Advantages and Limitations

   



   

Applications

1. Protein and Nucleic Acid Research

Polarized Raman Spectroscopy Analytical Service can be used to analyze the orientation and conformational changes of biomacromolecules, helping to reveal their structural symmetry and folding characteristics.

   

2. Cell and Tissue Characterization

By detecting molecular arrangements and local structural differences in micro-regions, the molecular environments in cells or tissues can be studied.

   

3. Polymer and Material Analysis

Polarized Raman Spectroscopy Analytical Service can be applied to polymer chain orientation, crystal structures, and microscopic characterization of functional materials, supporting performance optimization.

  

4. Nanomaterial and Crystal Research

Applied to the analysis of symmetry and orientation distribution of nanomaterials and crystalline samples, providing a basis for the development of new materials.

  

FAQs

Q1: What Is the Difference between Polarized Raman Spectroscopy and Conventional Raman Spectroscopy?

A1: Conventional Raman spectroscopy mainly provides information on molecular vibration modes and chemical bonds, while polarized Raman spectroscopy, by analyzing scattering signals at different polarization angles, can also reveal molecular symmetry, orientation, and conformational characteristics.

  

Q2: Is it Suitable for Quantitative Analysis?

A2: Polarized Raman spectroscopy is more suitable for qualitative and semi-quantitative studies. Orientation distribution can be reflected through peak intensity ratios, but in complex systems, additional techniques are still needed for validation.

  

Q3: What Factors May Affect the Accuracy of the Results?

A3: Uneven samples, background fluorescence interference, excessive surface roughness, the presence of impurities, or improper laser power may all affect the results. Therefore, sample preparation and control of experimental conditions are essential.