Plasma Proteomics Service

Plasma proteomics is the field that studies the composition, changes, and interactions of all proteins in plasma. Plasma is an important component of blood, and plasma proteins are a class of significant biomolecules found in plasma, primarily including albumin, globulins, and others. These proteins are involved in maintaining fluid balance, immune responses, substance transport, and regulating blood coagulation, among other physiological functions. Plasma proteomics reflects the body's health status and disease changes. Using high-throughput mass spectrometry, plasma proteomics can comprehensively detect and quantify thousands of proteins in plasma, revealing their variations in different physiological and pathological states.

 

Service at MtoZ Biolabs

MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. We have established a powerful and professional plasma proteomics platform including Thermo Fisher Q Exactive HF and Obitrap Fusion Lumos mass analyzer system, coupled with Nano-LC system. Our plasma proteomics service can be used in clinical research and biomarker discovery, providing the most comprehensive support for our clients. If you are interested in our service, please contact us freely.

 

Analysis Workflow

The analytical workflow for plasma proteomics service typically involves in sample collection and preparation, protein extraction and enrichment, digestion, peptide separation and MS analysis.

 



Figure 1. The Workflow of Plasma Proteomics

 

Service Advantages

1. High Sensitivity and Broad Coverage: MtoZ Biolabs employs advanced mass spectrometry technology and optimized experimental workflows to significantly enhance the detection sensitivity of low-abundance proteins in plasma. Using high-performance instruments and established analytical methods, we aim to providing reliable data support for plasma proteomicsservice accurately covering key proteins in human plasma across a wide range of samples.

 

2. Enhanced Reproducibility and Comparability: MtoZ Biolabs' plasma proteomics service emphasizes experimental standardization and data reproducibility. Through refined sample processing techniques and carefully controlled experimental conditions, we ensure high comparability of results, addressing inconsistencies between different studies. This approach improves protein coverage across studies and platforms, advancing clinical research and personalized medicine.

 

3. Customized Services: MtoZ Biolabs offers flexible, tailored plasma proteomics service designed to meet the specific research needs of our clients. Our workflows and solutions are optimized according to your research goals, ensuring high sensitivity, reproducibility, and comprehensive results in your experiments.

 

4. Professional Team: Our team consists of experienced scientists, bioinformaticians, and technicians with deep expertise in mass spectrometry, proteomics, and data analysis. With a strong academic and clinical research background, our team members are well-equipped to handle complex research challenges in plasma proteomics serviceand provide precise analyses and actionable research outcomes.

 

Case Study

1. Plasma Proteomics Identifies Proteins and Pathways Associated with Disease

This study utilized plasma proteomics from 46,165 UK Biobank participants to identify 157 proteins associated with incident depression over a 14.5-year follow-up. Key findings included associations between GDF15 and PLAUR with increased depression risk, while LRRN1 and ITGA11 were linked to decreased risk. Dysregulated proteins were correlated with brain regions involved in depression, such as the hippocampus, and stress-related events like trauma and self-harm. Pathway enrichment analysis revealed the immune response's critical role, with EGFR and TNF as central proteins. BTN3A2 was identified as a causal factor through Mendelian randomization, providing insights into depression’s development and potential interventions. Plasma proteomics service offers valuable insights into the molecular changes associated with disease. By analyzing plasma proteins, researchers can identify biomarkers of various disease, helping to better understand the condition's development and potential interventions.

 



Kang, J. et al. Sci Bull (Beijing). 2024.

Figure 2. Plasma Proteomics Identifies Proteins and Pathways Associated with Disease

 

2. Plasma Proteomics Unveils Endogenous Serum Peptides as Potential Disease Biomarkers

This study identified potential biomarkers for hepatocellular carcinoma (HCC) using endogenous peptidomics and bioinformatics analysis. Serum samples from 40 subjects (20 HCC cases and 20 liver cirrhosis patients) revealed 67 differentially expressed peptides, including three (VMHEALHNHYTQKSLSLSPG, NRFTQKSLSLSPG, SARQSTLDKEL) that outperformed AFP in diagnostic accuracy. The peptide IAVEWESNGQPENNYKT was uniquely detected in all HCC cases and absent in cirrhosis patients, highlighting its diagnostic potential. Gene ontology and pathway analysis suggested these dysregulated peptides may play roles in HCC development, offering valuable insights for improved diagnostic and therapeutic strategies. Plasma proteomics service can provide comprehensive analysis of serum samples to identify differentially expressed peptides and potential disease biomarkers, supporting early detection and diagnosis of conditions such as hepatocellular carcinoma.

 



Sajid, MS. et al. J Proteome Res. 2024.

Figure 3. Plasma Proteomics Unveils Endogenous Serum Peptides as Potential Disease Biomarkers

 

FAQ

Q1：In plasma proteomics, under what circumstances is it necessary to remove high-abundance proteins?

Answer: Situations where high-abundance proteins need to be removed in plasma proteomics:

1. Enhancing the detection sensitivity of low-abundance proteins: High-abundance proteins (such as albumin and immunoglobulins) can mask the signals of low-abundance proteins, making it difficult to detect and quantify the latter.

 

2. Studying specific biomarkers: If the focus of the research is on certain low-abundance biomarkers or disease-associated proteins, removing high-abundance proteins helps to focus on the target proteins.

 

3. Improving data reproducibility and accuracy: Removing high-abundance proteins can reduce data noise, enhancing the reliability of the analysis results.

 

4. Optimizing sample separation and analysis methods: Removing high-abundance proteins simplifies sample processing, improving the efficiency of subsequent separation and analysis.

 

Plasma proteomics services can be tailored and optimized based on specific client requirements and experimental conditions, including strategies such as removing high-abundance proteins to enhance the detection and analysis of target proteins.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Plasma Proteomics

5. Mass Spectrometry Image

6. Raw Data