Overpressure Solutions in Circular Dichroism Experiments

    The phenomenon of overpressure in Circular Dichroism (CD) experiments usually refers to the excessive pressure generated in the sample pool or system. This situation may lead to damage to the sample pool, leaks or sample deterioration, affecting the accuracy of the experimental results. 

     

    Factors Related With Overpressure

    1. Formation of Bubbles in the Sample Pool

    If the operation is improper when loading the sample, bubbles may easily form in the sample pool, causing increased pressure.

     

    2. High Viscosity of the Sample

    High viscosity samples may cause increased pressure during flow.

     

    3. Blockage of the Sample Pool

    Contaminants, sediments, or crystals in the sample pool or pipes may cause blockage, leading to overpressure.

     

    4. Temperature Control Issues

    Excessive temperature rise may cause the sample to expand, increasing pressure.

     

    Solutions to Overpressure

    1. Handling Carefully to Avoid Bubbles

    Ensure meticulous handling when loading samples to prevent bubble formation.

     

    2. Adjusting Sample Viscosity

    For highly viscous samples, reduce viscosity by dilution or temperature adjustment.

     

    3. Regular Cleaning and Maintenance of Sample Cell

    Keep the sample cell and pipelines clean, regularly check for and remove potential blockages.

     

    4. Control Experimental Temperature

    Ensure a stable temperature during the experiment to avoid overheating.

     

    If overpressure occurs, the first step is to confirm the cause, and then apply the above solutions accordingly.

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