Ononin Analysis Service

Ononin is an isoflavone glycoside compound belonging to the flavonoid class of plant secondary metabolites. Ononin is widely found in various leguminous plants, such as red clover (Trifolium pratense) and soybean (Glycine max). Additionally, it is also present in some traditional medicinal plants, such as licorice (Glycyrrhiza) used in traditional medicine. The chemical structure of ononin consists of an isoflavone core formed by a 4-oxo-benzopyranone ring and a benzene ring, to which a glycoside chain is attached. Its molecular formula is C₂₂H₂₂O₉.

In the body, ononin is primarily hydrolyzed and metabolized into its aglycone form, Formononetin. First, in the gastrointestinal tract, the glycosidic bond of ononin may be cleaved by hydrolase enzymes, releasing Formononetin. Subsequently, Formononetin can undergo further metabolic transformations into other metabolites, such as dihydroformononetin or its sulfate and glucuronide conjugates. These metabolites are typically excreted in the urine.

Figure 1. The Structure of Ononin

Ononin is an isoflavone glycoside that can be found in a variety of medicinal plants, showing potential antioxidant, antibacterial, and anti-inflammatory activities. MtoZ Biolab's Ononin Analysis Service provides a comprehensive ononin analysis, utilizing our advanced high-performance liquid chromatography-mass spectrometry (HPLC-MS) platform and the expertise of our metabolomics team.

Analysis Workflow 

Service Advantages

• Rapid Analysis Cycle and Cost-Effectiveness
• Stable, Reliable, and Highly Sensitive Analysis Results
• Comprehensive One-Stop Service for Extensive Testing Needs
• User-Friendly Operation with Wide Application Scope

Sample Submission Requirements

1. Sample Types: Serum, plasma, urine, tissue homogenates, and other biological samples.

2. Sample Volume: At least 200 μL of liquid sample or 200 mg of solid sample.

3. Sample Preservation: Store at -80°C to maintain stability.

Note: Provide details on sample collection and handling.

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Ononin

4. Raw Data

5. Custom Analysis Report

By offering this ononin analysis service, MtoZ Biolabs aims to facilitate your research and product development processes by providing accurate and reliable data on ononin.