Olink Proteomics Service
- High specificity: Proprietary PEA technology minimizes false positives with dual-antibody recognition.
- High sensitivity: Detection thresholds as low as fg or pg/mL.
- High throughput: Enables simultaneous detection of dozens to thousands of proteins.
- Minimal sample requirement: Requires as little as 1 µL, suitable for serum, plasma, and other biological fluids.
- Uncover molecular mechanisms and pathways underlying diseases.
- Identify potential therapeutic targets to streamline drug development.
- Discover novel biomarkers for diagnostics or prognosis.
- Support clinical trials with biomarker validation.
- Integrate multi-omics data for comprehensive biological insights.
Olink proteomics is an advanced high-throughput protein analysis technology based on proximity extension assay (PEA), combined with qPCR or NGS detection. This platform enables precise quantification and characterization of nearly 1,000 proteins using only 1 µL of sample, offering unmatched sensitivity, specificity, and efficiency.
Leveraging this cutting-edge technology, MtoZ Biolabs provides high-precision olink proteomics service, driving breakthroughs in disease mechanism studies, biomarker discovery, drug development, and personalized medicine. Through a professional team and state-of-the-art equipment, our olink proteomics service delivers comprehensive, reliable solutions to uncover dynamic changes in protein networks, bridging the gap between research insights and clinical applications.
Technical Principles
At the core of the olink proteomics platform is PEA, a biochemical technology that combines the specificity of antibodies with the sensitivity and amplification capacity of PCR. PEA technology utilizes two proximity probes, each consisting of an antibody linked to a unique DNA oligonucleotide. When the probes bind to their target protein, they come into close proximity, allowing the DNA oligonucleotides to hybridize and form a double-stranded DNA molecule that can be extended by DNA polymerase.
Unique sample indices are added during the subsequent PCR step, enabling efficient sample multiplexing. The resulting DNA sequences are directly quantified using real-time PCR or NGS, transforming protein presence into measurable DNA signals. With this approach, the NGS read counts are proportional to protein concentrations in the sample, allowing for accurate relative quantification.
Smith, J. G. et al. Circulation. 2017.
Figure 1. The Principle of PEA
Technical Advantages
Services at MtoZ Biolabs
MtoZ Biolabs offers one-stop olink proteomics service, supporting clients from experimental design to data interpretation while adhering to rigorous quality and data standards to maximize research outcomes.
1. Custom Project Design
Our technical experts tailor project plans to meet your research objectives, sample types, and analytical needs, offering feasibility assessments to ensure a clear research path.
2. High-Quality Sample Processing
Rigorous protocols for sample handling, including serum, plasma, cerebrospinal fluid, tissue extracts, and cell lysates, maintain the highest sample quality and stability throughout the entire process.
3. Precision PEA Assays and Detection
Automated platforms and experienced teams implement high-throughput, ultra-sensitive multiplex protein detection using Olink PEA technology, ensuring reproducible and accurate data through stringent quality control measures.
4. Comprehensive Data Analysis
Our bioinformatics team processes raw data with thorough cleaning, normalization, and statistical analysis, offering intuitive visualizations, differential protein screening, pathway enrichment, and network analysis to uncover biological insights.
5. Value-Added Support
We provide comprehensive project communication and value-added services, including follow-up experimental design advice, validation strategy recommendations, and multi-omics integration analysis, ensuring clients maximize the scientific and industrial impact of their research.
Bao, X. H. et al. Front. Mol. Neurosci. 2023.
Figure 2. The Workflow for Olink Proteomics
Why Choose MtoZ Biolabs?
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced olink proteomics platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all olink proteomics data, providing clients with a comprehensive data report.
4. Multi-Sample Compatibility: Our platform accommodates various biological samples—from purified proteins to complex matrices like cell lysates, tissues, and bodily fluids—to meet diverse research needs.
5. Customized Service: Tailored to the specific research needs of our clients, we offer flexible experimental design and personalized data analysis to ensure the achievement of research goals to the fullest extent.
Applications
Case Study
Case1
This study utilized olink proteomics to identify distinct immune response proteins in patients infected with non-tuberculous mycobacteria (NTM) and Mycobacterium tuberculosis (MTB) and unveiled unique molecular characteristics of each infection.
Wang, L. et al. Inflamm. Res. 2024.
Case2
Researchers leveraged olink proteomics to identify novel diagnostic biomarkers for dilated cardiomyopathy and heart failure which advanced early screening and precision medicine for cardiovascular diseases.
Xu, S. et al. J. Proteome Res. 2024.
FAQ
Q1: What advantages does olink Proteomics offer over traditional methods?
Olink proteomics provides ultra-sensitive, multiplex protein quantification with minimal sample input. It excels in detecting low-abundance proteins and offers greater simplicity, standardization, and reproducibility than traditional mass spectrometry, making it ideal for biomarker discovery and clinical research.
Choose MtoZ Biolabs for olink proteomics service to accelerate innovation and achieve impactful research outcomes!
How to order?
