Olink Assay Service
The Olink Assay is a cutting-edge proteomic technology designed to enable highly multiplexed and sensitive protein detection. At its core, the Olink Assay utilizes the Proximity Extension Assay (PEA) methodology, which relies on pairs of antibodies linked to unique DNA strands. When these antibody-DNA conjugates bind to their target proteins in a sample, the proximity of the DNA strands allows them to hybridize and be extended by a DNA polymerase, forming a unique DNA sequence that serves as a precise identifier for each protein. This innovative approach ensures exceptional specificity and sensitivity, enabling the simultaneous quantification of hundreds of proteins from minimal sample volumes. The robust nature of the Olink Assay makes it an invaluable tool for both exploratory research and targeted validation studies in diverse biological contexts.
Figure 1. Overview of the Olink Assay
The Olink Assay offers a multitude of applications that significantly advance proteomic research. The Olink Assay enables high-throughput protein profiling, facilitating comprehensive biomarker discovery essential for understanding disease mechanisms and developing targeted therapies. Additionally, the Olink Assay excels in clinical research settings, where it aids in patient stratification, monitoring disease progression, and evaluating therapeutic responses with unparalleled precision. Beyond these applications, the Olink Assay supports systems biology approaches by providing detailed insights into protein networks and interactions, thereby enhancing our understanding of complex biological systems. The versatility and reliability of the Olink Assay make it a preferred choice for researchers aiming to achieve depth and breadth in their proteomic analyses.
Service at MtoZ Biolabs
MtoZ Biolabs offers the Olink Assay Service to provide researchers with a wide range of Olink assay panels tailored to specific research needs, including inflammation, oncology, cardiometabolic panels, and more. By leveraging our expertise in mass spectrometry and protein chemistry, the Olink Assay Service ensures precise and reliable data generation. Furthermore, MtoZ Biolabs offers comprehensive support, from experimental design and sample preparation to data analysis and interpretation. Our commitment to quality assurance and adherence to industry standards guarantees that clients receive actionable insights that drive scientific discovery and innovation. Whether you are conducting large-scale biomarker studies or exploring intricate protein interactions, the Olink Assay Service by MtoZ Biolabs is designed to enhance the depth and breadth of your proteomic research.
Analysis Workflow
1. Antibody Pair Design
Specific antibody pairs are carefully selected and conjugated with unique DNA tags to ensure precise recognition of target proteins. Each antibody pair is optimized for high specificity and minimal cross-reactivity.
2. Sample Preparation
Biological samples, such as plasma, serum, or tissue lysates, are prepared without the need for depleting high-abundance proteins or enriching low-abundance ones, owing to the high sensitivity and specificity of the Olink Assay platform.
3. Incubation
Samples are incubated with antibody-DNA conjugates. When both antibodies bind to their target protein, the DNA tags are brought into proximity, enabling hybridization and extension to convert protein signals into nucleic acid signals.
4. Detection
The resulting DNA sequences are amplified and quantified using high-throughput techniques, such as qPCR or NGS, to ensure precise and reliable protein detection.
5. Bioinformatics Analysis
The Olink Assay data are processed through advanced bioinformatics tools, decoding DNA-based signals to identify and quantify target proteins. Results include protein abundance, differential expression analysis, and biological pathway insights.
Service Advantages
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Olink Assay Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Stop Service: MtoZ Biolabs provides one-stop service from antibody pair design, sample preparation to data analysis, saving your time and efforts.
3. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
4. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Olink Assay Service data, providing clients with a comprehensive data report.
Case Study
Plasma Proteomics Analysis of COVID-19 Patients Using Olink Assay
In a study of 306 COVID-19 patients and 78 symptomatic controls, the Olink Proximity Extension Assay (PEA) was utilized to profile 1,472 plasma proteins, revealing critical insights into disease mechanisms and progression. By leveraging the high-throughput and sensitivity of the Olink Assay, researchers identified significant upregulation of interferon (IFN) pathway proteins and chemokines such as IFN-γ, CXCL10, and CXCL11 in COVID-19 patients (Figure C–E). These proteins formed a clear disease-specific signature, distinguishing COVID-19 patients from controls and correlating with clinical severity. Through integration of proteomic data with single-cell RNA sequencing (scRNA-seq), the Olink Assay enabled precise mapping of elevated plasma proteins to immune cell origins, including T cells, monocytes, and plasmacytoid dendritic cells (Figure F). This demonstrated the assay's ability to uncover the cellular contributions driving immune dysregulation. The results showcased how Olink Assay facilitates comprehensive biomarker discovery and immune pathway analysis, providing a powerful tool for understanding disease mechanisms, identifying prognostic markers, and informing targeted therapeutic strategies for severe COVID-19.
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