NMR Hydrogen Spectrum Analysis of Conjugate Vaccine Carrier Proteins

Conjugate vaccines, also known as conjugated vaccines, such as the pneumococcal vaccine, belong to a special class of vaccines. The working principle of conjugate vaccines involves linking small-molecule antigens (usually polysaccharides) to large-molecule carrier proteins, which typically trigger a strong immune response. Administering a conjugate vaccine not only generates a substantial amount of antibodies but also enhances the effect of previous doses upon subsequent vaccinations, resulting in faster antibody production, higher antibody concentrations, and stronger protective effects. Conjugate vaccines are now widely used in medical applications, including children's immunizations, influenza vaccines, and HPV vaccines.

 

Nuclear magnetic resonance (NMR) hydrogen spectroscopy is a method based on the phenomenon of hydrogen atomic nuclei absorbing energy through resonance in a magnetic field. It is used to analyze the internal structure and properties of compounds. In the analysis of free polysaccharides in conjugate vaccines, NMR can provide detailed information about the structure of the polysaccharides, including the types of sugar residues, their linkage patterns, and sequences. Additionally, based on the intensity of the NMR spectrum, the quantity of free polysaccharides can be measured quantitatively.

 

NMR has many advantages in the analysis of free polysaccharides in conjugate vaccines. First, it is a non-destructive analytical method that does not require complex sample pretreatment or derivatization, allowing the sample to remain in its original state. Second, it provides detailed and accurate information about polysaccharide structures, aiding in the understanding of the interaction between polysaccharides and carrier proteins, as well as the mechanism of free polysaccharide formation. Additionally, it offers high sensitivity and resolution, enabling both qualitative and quantitative analysis of polysaccharides in complex samples.

 

MtoZ Biolabs provides one-stop NMR hydrogen spectroscopy analysis services for conjugate vaccine carrier proteins. Simply inform us of your experimental goals and send your samples, and MtoZ Biolabs will handle all subsequent project tasks, including sample preparation, analysis, data interpretation, and reporting. Feel free to contact us for a consultation at no cost.