N6,2'-O-dimethyladenosine Analysis Service
- Identifying the presence and level of N6,2'-O-dimethyladenosine in your samples.
- Understanding the impact of this modification on RNA stability and translation.
- Exploring the role of N6,2'-O-dimethyladenosine in disease pathogenesis and potential therapeutic targets.
- Expertise: In-depth knowledge in mass spectrometry and metabolomics, backed by a team of experienced scientists.
- Advanced Technology: Utilizing top-tier LC-MS/MS equipment for accurate and reliable results.
- Comprehensive Analysis: Detailed report including experimental procedures, MS/MS instrument parameters, and results interpretation.
- Customer Service: Providing personalized assistance and consultation for each project.
- Molecular Biology
- Epigenetics
- Drug Discovery
- Disease biomarker identification
- Samples should be stored at -80℃ and shipped with dry ice.
- We recommend providing at least 3 samples of similar conditions for each type of sample.
- Please provide details on sample collection and handling.
- Other sample types, please contact us for more details.
N6,2'-O-dimethyladenosine (m6Am) is a naturally occurring modified nucleoside found predominantly in eukaryotic messenger RNA (mRNA). Structurally, m6Am is characterized by the methylation of the nitrogen at the 6th position of adenine (N6) and an additional methyl group attached to the 2'-hydroxyl group of the ribose sugar. This dual methylation confers unique chemical properties to the nucleoside, influencing its stability and interaction with proteins involved in mRNA regulation. m6Am is most commonly found at the 5' cap structure of mRNAs, immediately following the 7-methylguanosine cap. This placement is crucial for regulating mRNA stability and translational efficiency.
Figure 1. The Structure of N6,2'-O-dimethyladenosine
m6Am has been shown to impact mRNA decay rates, translation initiation, and cellular stress responses. The methylation status of m6Am is tightly controlled by specific methyltransferases and demethylases, with enzymes such as PCIF1 catalyzing the methylation of adenosine, while FTO has been identified as a key demethylase. Dysregulation of m6Am levels has been implicated in various diseases, including cancer and metabolic disorders, making it a target of interest in both basic and clinical research.
Understanding the dynamics of m6Am in mRNA can reveal crucial details about gene expression regulation, offering valuable insights for fields such as oncology, epigenetics, and molecular biology. The ability to comprehensively analyze m6Am is thus vital for researchers aiming to unravel its complex biological roles and implications in health and disease.
At MtoZ Biolabs, we offer a comprehensive N6,2'-O-dimethyladenosine analysis service, utilizing advanced mass spectrometry techniques, to provide accurate, reliable and comprehensive details about m6Am modification in your samples. Our service is designed to solve the problems of:
Analysis Workflow
Service Advantages
Applications
This service is applicable in various research areas, including but not limited to:
Sample Submission Requirements
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on N6,2'-O-dimethyladenosine
4. Raw Data
5. Custom Analysis Report
At MtoZ Biolabs, we are committed to providing the best services to our clients for their research and development needs. For more details about our N6,2'-O-dimethyladenosine Analysis Service, please feel free to contact us.
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