N2,N2,7-trimethylguanosine Analysis Service

N2,N2,7-trimethylguanosine is a specific form of modified RNA nucleoside. It is found abundantly in eukaryotic tRNA and plays a crucial role in the stabilization of the tertiary structure of tRNA. N2,N2,7-trimethylguanosine is also involved in RNA processing events and mRNA export from the nucleus to the cytoplasm. Structurally, N2,N2,7-trimethylguanosine contains three methyl groups, with two at the nitrogen atom in the 2-position and one at the 7-position of the guanosine base. N2,N2,7-trimethylguanosine plays critical roles in biological processes. Aberrations in methylation patterns of RNA nucleosides, including N2,N2,7-trimethylguanosine, are associated with a broad spectrum of human diseases such as cancer, cardiovascular disease, and neurological disorders. Therefore, understanding the distribution and alteration of N2,N2,7-trimethylguanosine can provide novel insights into disease pathogenesis and potential therapeutic targets.

 



 

Figure 1. The Structure of N2,N2,7-trimethylguanosine

 

MtoZ Biolabs is proud to offer a specialized N2,N2,7-trimethylguanosine Analysis Service. This service is tailored to meet the demands of researchers seeking accurate and reliable quantification of this important nucleoside. Our service offerings include:

• Quantification of N2,N2,7-trimethylguanosine Levels: Using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS), we provide precise quantification of N2,N2,7-trimethylguanosine in various biological samples.
• Metabolic Profiling: Analyzing metabolic pathways involving N2,N2,7-trimethylguanosine to elucidate its role in cellular processes.
• Structural Characterization: Detailed examination of N2,N2,7-trimethylguanosine derivatives to understand their biological implications.
• Comparative Analysis: Assessing N2,N2,7-trimethylguanosine levels across different conditions or treatments to identify potential biomarkers.

 

Analysis Workflow

 



 

Service Advantages

• Extensive expertise in handling various biological samples and extracting metabolites.
• State-of-the-art mass spectrometry platforms.
• Strict quality control to ensure reliable results.
• Comprehensive statistical and bioinformatics analysis.
• Personalized service to meet various research needs.

 

Applications

Our N2,N2,7-trimethylguanosine analysis service can be applied in several fields:

• Basic Biological Research: Understanding the role and regulation of N2,N2,7-trimethylguanosine in RNA biology.
• Disease Research: Investigation of N2,N2,7-trimethylguanosine alterations in various human diseases.
• Drug Discovery: Evaluating the effect of drug candidates on N2,N2,7-trimethylguanosine levels.

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues, and other biological samples. It is recommended to prepare at least 3 biological replicates for each sample type.

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Storage and Shipping

Samples should be stored at -80℃ and shipped with dry ice. Avoid repeated cycles of freezing and thawing

 

Note: Please provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on N2,N2,7-trimethylguanosine

4. Raw Data

5. Custom Analysis Report

 

MtoZ Biolabs is dedicated to providing top-tier analytical services to assist with your research needs. Please contact us to find out more about how our N2,N2,7-trimethylguanosine Analysis Service can advance your research goals.