N-Terminal Sequencing (N-Terminal Unblocked) Service

N-terminal sequencing is a key technique in protein sequence analysis, particularly for identifying the N-terminal amino acids. Despite the advancements in mass spectrometry, Edman degradation remains an irreplaceable method due to its precision in determining N-terminal sequences. MtoZ Biolabs offers specialized N-terminal sequencing services, providing researchers with high-quality protein sequence information to support biopharmaceutical and life science research.

Edman degradation sequentially labels and cleaves N-terminal amino acids without disrupting other peptide bonds, revealing the protein's N-terminal sequence. This method is especially valuable for verifying the N-terminal boundaries of recombinant proteins or determining the N-termini of protease-resistant domains. It is also useful for analyzing new proteins and peptides not referenced in sequence databases.In biopharmaceuticals, N-terminal sequencing is essential for ensuring the quality and consistency of therapeutic proteins. Understanding the N-terminal sequence aids in determining protein structure, function, and stability. This is particularly important in the development of recombinant proteins, antibodies, and vaccines.

MtoZ Biolabs employs advanced Edman sequencing systems combined with automated PITC chemistry to deliver precise N-terminal protein sequencing. Our services adhere to GLP/cGMP standards and comply with ICH guidelines and FDA regulations. For high-quality, comprehensive N-terminal sequencing services, contact MtoZ Biolabs. Our expert team and advanced technology ensure precise and reliable results, supporting your research and development needs.

Technical Advantages

1. High Coverage

We achieve complete sequence coverage through multiple protease digestions and efficient HPLC-MS/MS technology, ensuring thorough detection of variations.

2. High Sensitivity

Our technology can detect very low levels of sequence variations, ensuring precise identification of minute mutations.

3. Professional Team

Our scientists and technicians have extensive expertise in mass spectrometry and proteomics, guaranteeing high-quality service.

4. Fast Turnaround

We provide rapid result delivery without compromising on quality, completing the analysis promptly.

Application

1. Protein Identification

By determining the N-terminal sequence of a protein, it can be accurately identified. This is particularly useful for the discovery and confirmation of new proteins.

2. Protein Modification Analysis

N-terminal sequencing can be used to detect and identify post-translational modifications (such as acetylation, methylation, etc.) of proteins, which is crucial for understanding protein function and regulatory mechanisms.

3. Biomarker Discovery

N-terminal sequencing can be used to identify and validate biomarkers, helping in the development of new diagnostic tools and therapeutic methods.

Submission Requirements

1. Sample Purity

The sample should be a high-purity protein, typically recommended to be above 90% purity, to reduce the interference of impurities on the sequencing results.

2. Sample Concentration

The sample concentration should be around 1 mg/mL to facilitate subsequent operations and processing. If the concentration is too low, it can be concentrated by methods such as centrifugation.

3. Sample Stability

The sample should be stored at low temperatures (usually -20℃ or -80℃) to prevent protein degradation. If the sample is prone to degradation, it is recommended to add appropriate protease inhibitors.