N,N-Dimethylguanosine Analysis Service

N,N-Dimethylguanosine is a methylated derivative of guanosine, characterized by the presence of two methyl groups (–CH₃) attached to the nitrogen atoms (N1 and N2) of the guanosine molecule. N,N-Dimethylguanosine is a common modification in RNA, particularly in non-coding RNAs such as tRNA and rRNA, where it is produced by the action of methyltransferases. It plays important roles in various biological processes, including cell metabolism, RNA processing, stability, and translation regulation.

 

Accurate quantification of N,N-dimethylguanosine is crucial for understanding its function in regulating gene expression, cell growth, and stress responses. Abnormal levels of N,N-dimethylguanosine may indicate dysregulation in RNA methylation processes, which are associated with certain cancers, neurodegenerative diseases, and other genetic disorders. Measuring N,N-dimethylguanosine levels in clinical and research settings can be used to assess the state of RNA modification and its changes under different pathological conditions, providing a basis for disease diagnosis and personalized treatment. The analysis of N,N-dimethylguanosine is typically performed using mass spectrometry-based techniques, such as liquid chromatography-mass spectrometry (LC-MS) or gas chromatography-mass spectrometry (GC-MS). These methods offer high sensitivity and specificity, enabling the accurate detection of N,N-dimethylguanosine in biological samples such as plasma, urine, or cell extracts.

 



Figure 1. The Structure of N,N-Dimethylguanosine

 

MtoZ Biolabs offers a state-of-the-art N,N-Dimethylguanosine Analysis Service, backed by our cutting-edge liquid chromatography-mass spectrometry (LC-MS) platform and an experienced team of metabolomics experts. Our service provides an in-depth analysis of N,N-dimethylguanosine, ensuring high sensitivity and precision.

 

Analysis Workflow

 



 

Service Advantages

1. Continuously refined protocols and cutting-edge analytical tools  

 

2. Expert experimental planning  

 

3. Rapid processing times  

 

4. Exceptional precision, selectivity, and sensitivity

 

Applications

• Qualitative analysis of the metabolites of N,N-dimethylguanosine.
• Quantitative analysis of N,N-dimethylguanosine content.
• Provide a basis for therapeutic improvement.

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues, and other biological samples. For each sample, it is necessary to select three or more materials under the same condition.

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Sample Preservation

Store at -80°C to maintain stability.

 

Note: Provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on N,N-Dimethylguanosine

4. Raw Data

5. Custom Analysis Report

 

At MtoZ Biolabs, we strive to facilitate your research and product development process by providing accurate and reliable results through our N,N-dimethylguanosine analysis service.