Mitochondrial Oxidative Phosphorylation Analysis Service
- ATP Synthase Activity
- NADH Dehydrogenase (Complex I) Activity
- Succinate Dehydrogenase (Complex II) Activity
- Cytochrome C Oxidase (Complex IV) Activity
- Coenzyme Q (CoQ) Reductase Activity
Mitochondrial oxidative phosphorylation is a core process of cellular energy production. Through the electron transport chain and ATP synthesis reactions, it converts chemical energy into usable cellular energy and is essential for maintaining cellular physiological functions. Mitochondrial oxidative phosphorylation analysis focuses on analyzing mitochondrial oxidative phosphorylation. By monitoring the oxygen consumption rate (OCR), this service effectively evaluates mitochondrial respiratory chain activity and ATP synthesis capacity, revealing the role of mitochondria in cellular energy metabolism.
The mitochondrial oxidative phosphorylation analysis service is widely applied not only in disease-related studies but also in plant research. In disease research, it helps uncover the impact of mitochondrial dysfunction on conditions such as diabetes, obesity, and Alzheimer’s disease, supporting the discovery of novel therapeutic targets. In plant research, it is used to assess mitochondrial function under various environmental conditions and to explore its essential role in energy supply, stress resistance, and plant growth and development.
Ghifari, A S. et al. Biochem SOC Trans, 2022.
Figure 1. Oxidative Phosphorylation (OXPHOS) Pathway in Plant Mitochondria.
Services at MtoZ Biolabs
Based on a high-resolution analytical platform, MtoZ Biolabs offers the mitochondrial oxidative phosphorylation analysis service focusing on analyzing mitochondrial oxidative phosphorylation. This service accurately assesses mitochondrial respiratory chain activity and ATP synthesis capacity, revealing their roles in cellular function, oxidative stress, and metabolic regulation. It helps researchers gain deep insights into the mechanisms of mitochondrial dysfunction, providing critical data support for studies on metabolic disorders, neurodegenerative diseases, and cancer. The main indicators that mitochondrial oxidative phosphorylation analysis can detect include:
1. Oxygen Consumption Rate (OCR)
By measuring the rate at which mitochondria consume oxygen, this analysis evaluates mitochondrial respiratory function and metabolic activity, reflecting the overall mitochondrial energy metabolism status.
2. ATP Production Capacity
This measures the ability of mitochondria to synthesize ATP under various conditions, revealing their energy production level and supporting the evaluation of cellular energy supply.
3. Oxidative Phosphorylation Enzyme Activity Assay
This assesses the activity of key enzymes involved in energy generation within mitochondria, revealing the overall function of the oxidative phosphorylation process. It includes, but is not limited to, the following enzyme activity measurements:
Analysis Workflow
1. Sample Preparation and Mitochondrial Isolation
Efficiently isolate mitochondria from cells or tissues, ensuring their purity and integrity for reliable analysis.
2. Functional Assessment and Testing
Evaluate mitochondrial performance in energy metabolism, ATP synthesis capacity, and respiratory chain activity by analyzing key indicators of mitochondrial function.
3. Data Collection and Processing
Utilize high-resolution technology to collect relevant data and perform multi-dimensional statistical analysis, ensuring the accuracy and comparability of the data.
4. Results Interpretation and Reporting
Provide a comprehensive interpretation of the oxidative phosphorylation process based on data analysis, revealing its role in cellular energy metabolism and supporting disease research and target discovery.
Sample Submission Suggestions
1. Sample Types
Applicable to animal cells, tissue samples, and isolated mitochondria. It is recommended to provide an adequate amount of samples to ensure the purity and representativeness of mitochondrial separation.
2. Sample Storage and Transport
Samples should be stored at −80°C immediately after processing and transported using dry ice to ensure cold chain transport. Avoid repeated freeze-thaw cycles to prevent mitochondrial dysfunction.
3. Sample Processing
Samples should avoid repeated freeze-thaw cycles. It is recommended to process and store samples as soon as possible to ensure mitochondrial activity and functionality. If partial preprocessing has been performed, please provide the relevant information.
Service Advantages
1. High Sensitivity and Precision
Based on advanced analytical platforms, the service precisely measures key mitochondrial functional indicators, ensuring high sensitivity detection and accurate quantitative analysis.
2. Comprehensive Functional Evaluation
The service covers a variety of mitochondrial functions, helping to reveal the critical role of mitochondria in cellular energy metabolism, and supporting diverse research needs.
3. High-Quality Data Output
Detailed and easy-to-interpret reports are provided, along with bioinformatics analysis, assisting researchers in understanding the functional changes of mitochondria under different physiological and pathological conditions.
4. Personalized Research Support
Customized experimental plans are provided based on the client’s specific research goals and needs, ensuring the service meets the unique requirements of various fields.
Applications
1. Metabolic Disease Research
Mitochondrial oxidative phosphorylation analysis service can be used to reveal mitochondrial dysfunction in metabolic diseases such as diabetes and obesity, helping to study the role of mitochondria in these diseases and their underlying mechanisms.
2. Cancer Research
By analyzing mitochondrial function in tumor cells, this service explores the impact of mitochondrial metabolic reprogramming on cancer development, supporting the discovery of new biomarkers and the development of targeted drugs.
3. Aging and Oxidative Stress Research
The service helps study the functional changes of mitochondria during aging, uncovering the connection between oxidative stress and aging, and supporting research on anti-aging and homeostasis regulation mechanisms.
4. Plant Research
Mitochondrial oxidative phosphorylation analysis service is also applicable to plant research, evaluating mitochondrial function in plants under various environmental stresses, and helping to understand the role of mitochondria in plant energy metabolism and stress resistance.
FAQ
Q1: Can Mitochondrial Oxidative Phosphorylation Analysis Be Combined with Other Omics Analysis?
A1: Yes. We offer multi-omics integrated analysis services, allowing for simultaneous mitochondrial proteomics, metabolomics, and other analyses, helping to deeply explore the multifaceted role of mitochondria in cellular functions.
Q2: Do You Support Analysis Across Multiple Conditions or Time Points?
A2: Yes. We can design multiple experiments based on research needs, comparing mitochondrial functional changes under different treatment conditions or time points, suitable for dynamic process monitoring and mechanism studies.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Data Analysis, Preprocessing, and Estimation
4. Bioinformatics Analysis
5. Raw Data Files
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