Lipidomics Pathway Analysis Service
Lipidomics pathway analysis is a comprehensive analytical approach that focuses on understanding the intricate biochemical pathways involving lipids within cells and tissues. Lipidomics pathway analysis aims to map the distribution, metabolism, and functional roles of lipids, revealing how they influence various cellular processes. Lipids are critical in maintaining cellular structure, energy storage, and signaling pathways. By combining advanced mass spectrometry with bioinformatics, lipidomics pathway analysis can accurately quantify and identify lipid species, trace metabolic changes, and assess lipid signaling networks, providing insights into their interrelationships and regulatory mechanisms in biological systems.
The application of lipidomics pathway analysis is crucial in understanding metabolic disorders, cardiovascular diseases, neurological conditions, and cancer. Lipidomics pathway analysis helps to uncover lipid-driven mechanisms that underpin disease progression and drug responses. It also aids in identifying novel biomarkers for early diagnosis and therapeutic targets, making it a vital resource for biomedical research and drug development.
Service at MtoZ Biolabs
Analysis Workflow
1. Sample Preparation: Biological samples are collected according to standardized protocols to ensure lipid preservation and integrity for accurate lipidomics pathway analysis.
2. Lipid Extraction: Lipids are extracted using specific solvents or extraction methods to ensure a comprehensive lipidome profile from complex biological matrices.
3. Mass Spectrometry Analysis: Lipid extracts are analyzed with high-resolution mass spectrometry (LC-MS), enabling precise identification and quantification of lipid species across various lipid classes.
4. Data Preprocessing: Raw data undergoes preprocessing, including denoising, baseline correction, peak alignment, and normalization, to prepare it for subsequent lipidomics pathway analysis.
5. Feature Extraction: Relevant lipid species are identified by locating peaks in the mass spectrometry data, with peak intensity corresponding to lipid abundance.
6. Statistical Analysis: Statistical methods like t-tests, ANOVA, and multivariate analysis are applied to identify significant differences in lipid profiles, facilitating in-depth lipidomics pathway analysis.
7. Identification and Annotation: Extracted features are annotated, matching lipid species with known databases to confirm their identity and classification, enabling precise mapping of lipid pathways in the biological context.
8. Pathway Analysis: Lipidomic data is mapped onto metabolic and signaling pathways to reveal lipid-mediated biological processes and their potential roles in disease.
9. Visualization: Comprehensive visualizations, such as pathway maps, volcano plots, and heatmaps, are generated to provide clear, actionable insights into lipidomic data.
Figure 1. The Workflow for Lipidomics Pathway Analysis Service
Service Advantages
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Lipidomics Pathway Analysis Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. Comprehensive Lipid Profiling: Enables the identification, quantification, and pathway analysis of lipid species across all major lipid classes, providing deep insights into lipid metabolism and cellular functions.
3. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
4. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all lipidomics pathway analysis data, providing clients with a comprehensive data report.
Case Study
Atherosclerosis (AS), a leading cause of cardiovascular disease, is closely associated with dyslipidemia, a key driver of its initiation and progression. This study explored the lipid profiles in the aortas of ApoE−/− mice fed a high-fat diet (HFD) and a normal diet (ND) using UHPLC-MS. Results revealed 131 differential lipids, including significantly increased levels of phosphocholines (PCs) and lysophosphocholines (LPCs) in the HFD group, highlighting their potential role in AS development. KEGG pathway enrichment analysis (Figure A, B) identified pathways associated with these altered lipids. Lipids with increased levels were enriched in glycerophospholipid and choline metabolism, while those with decreased levels were linked to fat digestion, insulin resistance, and thermogenesis. Further analysis of the glycerophospholipid metabolism pathway (Figure C) revealed elevated levels of lipids such as phosphatidyl-L-serine and phosphatidylcholine, suggesting their critical role in AS progression. These findings demonstrate how lipidomics pathway analysis can uncover key metabolic pathways and lipid biomarkers, providing valuable insights into the mechanisms driving atherosclerosis.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
At MtoZ Biolabs, we are committed to providing high-quality, reliable, and personalized analysis services. For more information about our Lipidomics Pathway Analysis Service, please contact us.
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