LC-MS Protein Analysis Service
The LC-MS Protein Analysis Service is an efficient solution that employs liquid chromatography-mass spectrometry (LC-MS) technology to achieve precise identification, quantitative analysis, and post-translational modification (PTM) characterization of proteins in complex biological samples. Proteins are the key executors of life activities, and their structure, function, and PTMs have profound impacts on biological processes. Comprehensive and in-depth proteomic analysis, particularly of protein composition and function in complex samples, is a significant challenge in modern life sciences research. Protein analysis is not only a fundamental tool in basic biological studies but also a crucial approach for disease mechanism research, drug target discovery, and biomarker screening.
Duracova M, et al. Toxins (Basel). 2018.
Figure1. LC-MS-based Methods in Protein-based Toxins Analysis
Liquid chromatography-mass spectrometry (LC-MS) provides superior separation capability and accuracy for protein analysis by integrating liquid chromatography (LC) with mass spectrometry (MS). LC primarily separates different sample components based on their interactions with the solvent and retention time. After separation, the eluted components are ionized using sources such as electrospray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI), converting them into gas-phase ions. These ions are then analyzed by the mass spectrometer, which measures their mass-to-charge ratio (m/z) to enable qualitative and quantitative analysis of proteins and peptides.
Services at MtoZ Biolabs
MtoZ Biolabs specializes in mass spectrometry-based proteomics, leveraging an advanced LC-MS platform including high-resolution Thermo Scientific Orbitrap series mass spectrometers and a team of skilled experts. We provide high-quality LC-MS protein analysis services whether for studying low-abundance proteins, complex post-translational modifications, or disease mechanisms. Our services are designed to meet your research needs and support scientific discoveries and innovative breakthroughs.
Our LC-MS-based protein analysis services extend beyond simple protein identification, offering high-precision quantitative analysis, peptide sequencing, and PTM characterization. We provide comprehensive and in-depth analytical solutions for your research:
1. Protein Identification and Quantification
Utilizing high-resolution LC-MS technology, we offer precise protein identification and quantitative analysis for complex biological samples. Whether analyzing a single protein or an intricate multiprotein system, our approach ensures reliable results.
2. Post-Translational Modification (PTM) Analysis
Our LC-MS technology enables detailed analysis of various PTMs, including phosphorylation, acetylation, glycosylation, and more. PTM characterization is essential for understanding protein roles in cellular signaling and functional regulation.
3. Protein Complex Analysis
By integrating LC-MS with crosslinking techniques, we provide composition analysis of protein complexes, helping to elucidate protein-protein interactions and network dynamics.
4. Peptide Sequencing
We offer peptide sequencing services using LC-MS/MS, delivering high-precision sequence information to help clients gain deeper insights into protein structure and function.
5. Labeled Quantitative Analysis (TMT, iTRAQ)
Through labeling technologies such as Tandem Mass Tag (TMT) and Isobaric Tags for Relative and Absolute Quantification (iTRAQ), we perform high-throughput quantitative analysis across multiple samples, making it ideal for large-scale comparative studies.
Rojas Echeverri, J. C. et al. Antioxidants (Basel). 2021.
Figure2. LC-MS-based Analysis of Plasma Samples
Why Choose MtoZ Biolabs?
✔ Cutting-Edge Mass Spectrometry – High-resolution LC-MS/MS ensures unmatched sensitivity and specificity.
✔ Customized Analysis Workflows – Tailored study designs for specific research needs.
✔ Advanced Bioinformatics Pipelines – Utilizing MaxQuant, Proteome Discoverer, and Skyline for high-confidence data interpretation.
✔ Integrated Multi-Omics Approach – LC-MS proteomics combined with transcriptomics, metabolomics, and interactomics.
✔ Expert Consultation & Support – Our team of mass spectrometry and bioinformatics specialists provides comprehensive guidance for your projects.
Sample Submission Suggestions
To ensure optimal results, we recommend the following sample submission guidelines:
✔ Protein Samples: ≥50 µg total protein per sample (cell lysates, tissue extracts, purified proteins)
✔ Peptide Samples: ≥10 µg digested peptides (trypsin, Lys-C, or other enzymes)
✔ Plasma/Serum Samples: Minimum volume of 50 µL
✔ Cell Culture Samples: At least 1 × 10⁷ cells per sample
✔ Affinity-Purified Samples: Provide IP/MS-compatible reagents or enrichment information
MtoZ Biolabs provides detailed sample submission guidelines and pre-experiment consultation to optimize study design.
At MtoZ Biolabs, we are committed to delivering high-impact LC-MS protein analysis solutions that drive scientific discoveries and innovations in proteomics, precision medicine, and pharmaceutical development. Contact us today to accelerate your LC-MS-based protein research!
How to order?
