Isolithocholic Acid Analysis Service

Isolithocholic acid (ILCA) is the 3β-hydroxy epimer of lithocholic acid (LCA) and is a secondary bile acid produced through the microbial conversion of primary bile acids in the intestine. While ILCA shares a similar chemical structure with LCA, its unique 3β-hydroxy group results in distinct biological activities and metabolic pathways. ILCA is recognized as a potential biomarker for various hepatobiliary and metabolic disorders, making the precise measurement of ILCA and its metabolites essential for disease diagnosis and therapeutic applications.

Figure 1. Molecular Structure of Isolithocholic Acid

MtoZ Biolabs offers Isolithocholic Acid Analysis Service using high performance liquid chromatography-mass spectrometry (HPLC-MS). This advanced method combines the separation capabilities of HPLC with the detection power of MS, ensuring high sensitivity and specificity in ILCA detection.

Analysis Workflow

Service Advantages

1. High Sensitivity and Precision

HPLC-MS/MS provides highly sensitive and accurate measurements, suitable for detecting low concentrations of ILCA.

2. Rapid and Efficient

Optimized sample preparation and fast analysis ensure quick and efficient processing.

3. Wide Applicability

Suitable for various biological samples, including blood, urine, feces, and tissues.

4. Accurate and Reliable Data

Ensures high reproducibility and low error rates, supporting both research and clinical applications, providing reliable and specific results.

Sample Submission Requirements

Make sure the sample volume is greater than 0.2 g or 0.2 mL. For detailed sample submission information, please contact our customer service.

Applications

1. Study ILCA's function in metabolic disorders, uncovering its role in metabolic imbalances.

2. Assess ILCA’s metabolic pathways to support the development of bile acid-related therapies.

3. Analyze ILCA levels to explore its role in conditions such as cholestasis, cirrhosis, and gallstones.

4. Investigate ILCA’s involvement in cholesterol metabolism, offering insights for the prevention and treatment of hypercholesterolemia.

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Isolithocholic Acid

4. Raw Data

5. Custom Analysis Report