Indole-3-Acetamide Analysis Service

    Indole-3-acetamide is an organic compound that belongs to the class of indole derivatives, specifically characterized by an acetamide group attached to the third position of the indole ring. It has a molecular formula of C10H10N2O and a molecular weight of 174.20 g/mol. This compound is a key intermediate in the biosynthesis of indole-3-acetic acid (IAA), an important plant hormone that regulates growth and development. Indole-3-acetamide has garnered interest in both plant biology and pharmacological research due to its role as a precursor in the biosynthetic pathway of IAA. In plants, it serves as an intermediate in the production of IAA, which influences various physiological processes such as cell division, elongation, and differentiation, as well as responses to environmental stimuli. Furthermore, studies suggest that indole-3-acetamide and its derivatives may possess antimicrobial, anti-inflammatory, and anticancer properties, potentially offering therapeutic benefits in clinical settings.


    The analysis and quantification of indole-3-acetamide in various samples can be performed using advanced chromatographic techniques such as high-performance liquid chromatography (HPLC) or gas chromatography (GC), often coupled with mass spectrometry (MS) to enhance sensitivity and specificity. These methods enable the precise detection and measurement of indole-3-acetamide at low concentrations, which is essential for studying its role in biological systems, understanding its metabolic pathways, and evaluating its potential applications in medicine and agriculture.

     

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    Figure 1. The Structure of Indole-3-Acetamide

     

    At MtoZ Biolabs, we utilize our state-of-the-art liquid chromatography-mass spectrometry (LC-MS) technology combined with our proficient metabolomics team to deliver an industry-leading Indole-3-Acetamide Analysis Service. This service ensures a thorough analysis of indole-3-acetamide, promising both high sensitivity and precision.

     

    Analysis Workflow

     

    1829023929571332096-WorkflowofIndole-3-AcetamideAnalysisService.PNG

     

    Service Advantages

    1. Advanced Technological Platform

    We use industry-leading mass spectrometry equipment and analysis techniques to ensure high precision and reliability of the analysis results.

     

    2. Extensive Experimental Experience

    With years of experience in experimental operations and data processing, we can handle various complex biological samples.

     

    3. Rapid Data Delivery

    Ensure quick sample processing and timely delivery of research results to facilitate scientific progress.

     

    Applications

    • Qualitative and quantitative analysis of indole-3-acetamide in various biological samples.
    • Determination of metabolic pathways of indole-3-acetamide.
    • Evaluating the role of indole-3-acetamide in biological processes and disease states.

     

    Sample Submission Requirements

    1. Sample Types

    Serum, plasma, urine, tissues and other biological samples. At least 3 identically conditioned samples are required for each sample type.

     

    2. Sample Volume

    • Animal tissue ≥ 100 mg
    • Plant tissue ≥ 200 mg
    • Serum/ Plasma ≥ 200 μL
    • Urine ≥ 2 mL

     

    3. Sample Preservation

    Samples should be stored at -80°C to maintain stability.

     

    Note: Detailed guidelines for sample collection and handling will be provided.

     

    Deliverables

    1. Experimental procedures

    2. Relevant liquid chromatography and mass spectrometry parameters

    3. Detailed information on Indole-3-Acetamide

    4. Raw data

    5. Custom analysis report

     

    Our Indole-3-Acetamide Analysis Service is designed to assist your research and product development efforts by providing accurate and reliable results.

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