Immune Phage Display Antibody Library Screening Service for Exosome
- B cells are isolated from immunized animals or human donors, and the variable regions (VH and VL) of antibody genes are amplified using PCR.
- The amplified genes are cloned into phagemid vectors, which allow the expression of scFv (single-chain variable fragment) or Fab (fragment antigen-binding) antibody formats on phage surfaces.
- The constructed antibody library is expressed in bacteriophages, enabling the presentation of antibody fragments on the phage coat proteins.
- Biopanning is performed to select phages displaying antibodies that bind specifically to exosomal surface markers. This involves multiple rounds of binding, washing, and elution to enrich high-affinity clones.
- The selected high-affinity antibody sequences undergo recombinant expression in bacterial or mammalian cell systems.
- Antibody affinity and specificity are validated using techniques such as ELISA, Surface Plasmon Resonance (SPR), and Flow Cytometry.
Immune Phage Display Antibody Library Screening Service for Exosome is an advanced platform for the discovery and screening of high-affinity antibodies targeting exosomal surface markers. This service utilizes phage display technology, an in vitro selection technique that allows the identification of antibodies with high specificity and affinity for exosome-related antigens. MtoZ Biolabs offers Immune Phage Display Antibody Library Screening Service for Exosome to facilitate the development of exosome-based diagnostics, therapeutics, and research applications by providing a robust pipeline for generating and screening high-quality antibodies.
Exosomes are small extracellular vesicles (EVs) secreted by cells, playing a crucial role in intercellular communication, disease progression, and biomarker discovery. These vesicles carry bioactive molecules such as proteins, lipids, and nucleic acids, making them valuable for studying various physiological and pathological processes. Exosome characterization primarily relies on their surface markers, which differ depending on the cellular origin and biological function of the exosomes. To efficiently study and manipulate exosomes, specific antibodies targeting exosomal surface markers (e.g., CD9, CD63, CD81, EpCAM, TSG101) are essential. The Immune Phage Display Antibody Library Screening Service for Exosome enables the selection of high-affinity antibodies against these markers, accelerating advancements in exosome-based applications such as biomarker identification, targeted drug delivery, and therapeutic exosome engineering.
Phage display antibody screening relies on diverse antibody libraries, ensuring a broad selection of potential antibody candidates. The key types of libraries used in our Immune Phage Display Antibody Library Screening for Exosome include:
💠Immune Libraries: Constructed from B cells of immunized animals or humans, these libraries contain antibodies that have undergone affinity maturation, resulting in high-affinity candidates.
💠Naïve Libraries: Derived from non-immunized donors, these libraries provide a diverse repertoire of antibodies, allowing for the selection of novel candidates even without prior antigen exposure.
💠Synthetic Antibody Libraries: Designed using rational engineering of complementarity-determining regions (CDRs), synthetic libraries introduce predefined amino acid diversity at critical binding sites, enabling the generation of customized antibodies with optimized properties. These libraries allow for greater control over sequence variability and are particularly useful when target-specific immune responses are difficult to elicit.
💠Semi-Synthetic Antibody Libraries: These libraries combine naturally derived CDRs with synthetic modifications to increase binding diversity. By introducing controlled sequence variations into the hypervariable regions, semi-synthetic libraries expand antibody diversity while retaining the structural stability and functional characteristics of naturally occurring antibodies.
Figure 1. Four Types of Antibody Libraries can be Distinguished by Source and Design
Analysis Workflow
1. Library Construction
2. Phage Display and Selection
Figure 2. Antibody Derived Fragments are Genetically Fused to Phage-coat Protein and Presented on the Surface of Filamentous Phage
3. Recombinant Antibody Production
Service Advantages
☑️Comprehensive Exosome Antibody Screening Platform: We offer a highly optimized phage display system for selecting high-affinity antibodies against exosomal surface markers, ensuring specificity and binding efficiency.
☑️Experienced Team of Experts: Our team has extensive experience in phage display, antibody engineering, exosome biology, and mass spectrometry, ensuring high-quality outcomes for every project.
☑️One-Stop Service: Our end-to-end workflow includes library construction, antibody screening, recombinant expression, and functional validation, streamlining the entire process.
☑️Customizable Solutions: We provide tailored solutions for biomarker discovery, exosome isolation, drug delivery, immunotherapy, and diagnostics, meeting diverse research and clinical needs.
Applications
1. Exosome Isolation & Purification
Provides antibodies for affinity-based exosome capture, improving research and clinical applications.
2. Biomarker Discovery
Enables high-affinity antibody selection for exosomal surface markers, supporting disease diagnostics and liquid biopsy.
3. Disease Mechanism Research
Identifies exosome-associated proteins to study cell signaling, disease progression, and intercellular communication.
4. Exosome-Based Drug Delivery
Provides antibodies for functionalizing exosomes to achieve targeted drug delivery.
With MtoZ Biolabs' cutting-edge technology and expertise, we provide highly specific, high-affinity antibodies for exosome-targeted research and therapeutic development. If you are interested in our Immune Phage Display Antibody Library Screening Service for Exosome, please feel free to contact us. Our technical specialists are available to provide a free business assessment.
How to order?
